Employing proteomics to understand the mechanisms of action of antipsychotics in human oligodendrocytes

Author(s)

Caroline Brandão Teles

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia
• Defense date: 2018-02-21

Examining board members

Daniel Martins de Souza; Lucilene Delazari dos Santos; Andre Schwambach Vieira

• Advisor: Juliana Silva Cassoli; Daniel Martins de Souza

Abstract

Schizophrenia is a chronic mental disorder that affects on average 0.7% of the world population, and its development is related to genetic and environmental factors. Cognitive disorders such as attention, memory and executive functions are often observed in patients with the disease. Antipsychotics consist of the main form of treatment and are widely used in the clinic. In the present project the proteome of a human oligodendrocyte cell line (MO3.13 cells) treated with four antipsychotics, two of the first generation (chlorpromazine and haloperidol) and two of the second generation (quetiapine and risperidone) were analyzed. The analysis of the treatments was performed by liquid nano-chromatography followed by tandem mass spectrometry (nano LC-MS / MS). Subsequently, the proteins found with p-value <0.05 were submitted to analysis in the Ingenuity Pathway Analysis (IPA) program to obtain the metabolic pathways related to each treatment. Canonical pathways were found in common and specific for each antipsychotic. The data generated in this dissertation helped to understand the biochemical pathways involved in the mechanism of action of these drugs, which may guide the development of new treatments (AU)

• FAPESP's process: 15/23049-0 - Employing human oligodendroglial cell cultures and glutamatergic dysfunction to understand the molecular basis of schizophrenia
• Grantee: Caroline Brandão Teles Rodrigues
• Support Opportunities: Scholarships in Brazil - Master