Selection of artificial carious lesion protocol and evaluation of physical-mechanical properties of resinous infiltrants before and after pH cycling

Author(s)

Gabriela Alves de Cerqueira

• Document type: Master's Dissertation
• Press: Piracicaba, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba
• Defense date: 2022-02-18

Examining board members

Giselle Maria Marchi; Lívia Aguilera Gaglianone; Fernanda Miori Pascon

• Advisor: Giselle Maria Marchi

Abstract

This study aimed to select a protocol of induction of artificial carious lesion for methodological standardization of other studies using resinous infiltrants of the research group; to evaluate the penetration depth of an experimental resinous infiltrant (composed of 75% triethylene glycol dimethacrylate - TEG, 25% bisphenol A ethoxylated diglycidyl methacrylate - Bis-EMA, 1% ethyl 4-dimethylaminobenzoate - EDAB and 0.5% camphorquinone) compared to the commercial infiltrant Icon® and, to analyze the surface roughness and microhardness of the resinous materials infiltrated in dental fragments before and after the pH cycling. To validate the caries induction protocols, fragments of sound third molars were used, divided into three groups (n=11): (I) 4 cycles, (II) 7 cycles and (III) 10 cycles, with demineralizing solutions, pH 4.4 and remineralizers, pH 7.0. Afterwards, with the exception of white caries spot, they were visually and according to the form according to the ICDAS code. Surface microhardness analysis (n=11) was performed before and after caries induction protocols in the three groups. And, qualitative images of white spot bonds were recorded using Transverse Microradiography (n=5). After the validation of the caries induction protocol, for the evaluation of resin infiltrants, new fragments of sound third molars were used. For a qualitative analysis of the penetration depth, the fragments were selected after 10 cycles of formation of the remineralizing units and divided into 2 groups (n=5): (I) Experimental Infiltrator (IE) and (II) Icon Commercial Infiltrator (IC). Confocal infiltration microscopy of infiltrated resins, following an infiltration of the manufacturer resin infiltration fragments, were applied to commercial infiltration confocal microscopy images. For the evaluation of surface roughness and microhardness, new fragments were distributed in two groups (n=20): (I) Experimental Infiltrator (IE) and (II) Commercial Icon Infiltrator (IC), and benefits to three different: (T1) stain lesion white caries, (T2) infiltrated resin material and (T3) infiltrated resin material exposed to pH cycling. The proposals were carried out in the R* program, with a significance level of 5%, except for qualitative variables. Lesions code ICDAS 2 (white stain noticeable with cleaning tooth surface) and ICDAS 3 noting cavitations without dentin exposure (protocol level only) were modeled from the protocol of the caries protocols of 4 cycles, 7 and 10, cycloremineralization. experimental reached values of surface roughness similar to the icon after separation and, its infiltration was maintained after reduced pH cycling. A 10-cycle caries induction protocol was selected. The experimental resin infiltrant was efficient in penetrating the white spot caries lesion and reducing surface roughness, however, it was not able to increase surface microhardness (AU)

• FAPESP's process: 19/13621-0 - Evaluation of the depth of resin infiltrant penetration in respect of three protocols for the validation of ariosial injury
• Grantee: Gabriela Alves de Cerqueira
• Support Opportunities: Scholarships in Brazil - Master