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Evaluation of the depth of resin infiltrant penetration in respect of three protocols for the validation of ariosial injury

Grant number: 19/13621-0
Support type:Scholarships in Brazil - Master
Effective date (Start): January 01, 2020
Effective date (End): December 31, 2021
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Giselle Maria Marchi Baron
Grantee:Gabriela Alves de Cerqueira
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


The aim of this study is to validate the protocol of induction of artificial carious lesion, having as variable factor the time of des- remineralization (DES-RE), for the methodological standardization of other studies with resinous infiltrants, to evaluate the depth of Penetration of an experimental resinous infiltrant when compared to the commercial Infiltrant Icon® and, analyze the microhardness and surface roughness of the material after establishment of the best protocol of carious lesion. In order to test the deep penetration of the experimental infiltrants (75% of TEGDMA, 25% of BisEMA, 0.5% of Canforoquinone (CQ) and 1% of EDAB) and commercial (Icon®) will be performed, initially, three des- remineralization protocols containing same substances (2, 2mMol/L CaCl2 + 2, 2mMol/L NaH2PO4 + 50mMol/acetic acid adjusted with NaOH at pH 5.0 E, 1, 5mMol/L Ca + 0, 9mMol/L P + 0, 05ppm F + 0, 1Mol buffer solution, pH 7.0), in three different times: 7, 14 and 28 days, thus simulating the oral environment. After the validation of the protocols and the evaluation of the depth of penetration, specimens infiltrated with resinous material will be submitted to the best protocol of artificial carious lesion, and then will be analyzed the microhardness and roughness Surface of the resinous material. The properties analyzed will be: enamel demineralization after des-remineralizing challenge through Microradiography (TMR) and optical microscopy by polarized light (MLP), penetration depth of infiltrants through confocal microscopy of Laser scanning (MCVL) e, Knoop superficial microhardness (KHN) and superficial roughness after the DES-RE Challenge of the best protocol of artificial carious lesion. The statistical analysis will be performed based on two-way ANOVA and Tukey's test with significance of 5%, for each quantitative test separately. (AU)