Obtaining synbiotic microparticles from xylo-oligosaccharides from sugarcane straw

Author(s)

Manoela Martins

• Document type: Master's Dissertation
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos
• Defense date: 2020-02-12

Examining board members

Rosana Goldbeck; Louise Emy Kurozawa; Taisa Magnani Dinamarco

• Advisor: Ana Carla Kawazoe Sato; Rosana Goldbeck

Abstract

Agroindustrial waste, besides being abundant, is a source of several components of industrial interest that can be explored and used to produce high value-added bioactive molecules in a cheap, renewable and sustainable way. Sugarcane straw generated during sugarcane processing is rich in xylan, susceptible to the action of xylanase enzymes for the production of prebiotic xyloligosaccharides, improving the viability of probiotic microorganisms in food. The present work aimed to apply the agroindustrial residue of sugarcane straw to the production of xylo-oligosaccharides through the action of recombinant Cryptococcus flavescens endoxylanase expressed in Pichia pastoris GS115, and study the association of these components in alginate and gelatin encapsulation matrices with probiotic bacteria. producing symbiotic microparticles. The main products of hydrolysis were xylobiose and xylotriose, short-chain XOS with the greatest prebiotic potential. The structural characteristic of the obtained matrices was evaluated by FT-IR, XRD, SEM and rheological tests. The microparticles obtained by ion gelation were evaluated for diameter and porosity, with a reduction in pore size and an increase in crystallinity with the addition of 3% XOS to the alginate-gelatin matrix, as well as intensification of molecular interactions. The viability of probiotics was analyzed by in vitro digestibility assays as well as refrigerated storage. The association of probiotics and XOS from sugarcane straw promoted greater survival of L. acidophilus throughout the storage period. In the digestibility assay, the alginate, gelatin and XOS 3% microgels were resistant to gastric conditions, and allowed the gradual release of the encapsulated organism in the intestinal environment, its site of action, with a reduction in viability of only 12.5%, and the microparticles without the addition of XOS had a drop of 31.55% in cells viability. The morphological and physical-chemical evaluations showed that the microgels were efficient for the protection of microorganisms, guaranteeing the survival and viability of the probiotics during the microencapsulation process, resistance to digestive fluids by in vitro simulation and storage stability (AU)

• FAPESP's process: 18/14223-5 - Obtaining symbiotic microcapsules of xylo-oligosaccharides from sugarcane straw
• Grantee: Manoela Martins
• Support Opportunities: Scholarships in Brazil - Master