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| Author(s): |
Tamires Pereira Dutra
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Piracicaba, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2022-02-17 |
| Examining board members: |
Enílson Antonio Sallum;
Karina Gonzales Silverio;
Poliana Mendes Duarte;
Joni Augusto Cirelli;
Marcio Zaffalon Casati
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| Advisor: | Enílson Antonio Sallum |
| Abstract | |
Despite the apparent similarities between gingivitis and peri-implant mucositis, differences in the clinical, inflammatory, and microbiological response may be present between teeth and implants and between patients with different periodontal histories. This study aimed to evaluate, through clinical, immunological (Multiplex) and microbiological (16S rRNA sequencing) approaches the changes that occur in the tissues during the experimental gingivitis and peri-implant mucositis model, comparing patients with a history of generalized aggressive periodontitis (Grade C periodontitis) (H-PerioC) and periodontally healthy individuals H-Health. Twenty individuals (n=10 per group), presenting at least one single tissue level implant, rehabilitated with a screw-retained single crown (more than 6 months in function) and a contralateral tooth in a position similar to the implant, were included. The subjects underwent abstention from oral hygiene for 21 days in the selected areas (tooth and implant), with a personalized stent. After this period, they resumed normal oral hygiene and were followed up to day 42. Clinical parameters, crevicular fluid, and subgingival biofilm were collected at baseline, during induction (7-14-21 days), and remission (42 days) of the inflammatory process. Clinically, teeth and implants in both groups responded to the experimental gingivitis/mucositis protocol with significant variation in all clinical parameters over time but with no significant difference when comparing teeth and implants and between groups (p> 0.05). Overall, none of the inflammatory and bones markers showed a statistically significant difference during the biofilm accumulation phase (0, 7, 14 e 21) at any site or group (p> 0.05). Comparisons between sites showed that, at baseline, the concentrations of IFN-?, IL-10, IL-17, IL-4, TNF-? were significantly higher in PICF (Peri-implant Crevicular fluid) compared to GCF (Gingival Crevicular Fluid), in the H-Health group (p < 0.05). During the biofilm accumulation phase, IFN-?, IL-10, IL-4 concentrations in implants remained significantly higher compared to teeth (p < 0.05). No significant differences were observed between tooth and implant in the H-PerioC group. Comparisons between groups showed that the concentrations of IFN-?, IL-1? IL-10, IL-17, IL-4, and TNF-? in the implants were higher in the H-Health group compared to the H-PerioC group (p <0.05). On the other hand, no significant difference was observed in teeth in the comparison between the H-PerioC and H-Health groups. Periodontal history revealed no significant impact on the microbiological response of teeth and implants throughout the induction/remission of inflammation. Significant changes in alpha diversity were observed only in teeth (both groups), whereas beta diversity varied significantly at both sites, however, changes over time in teeth were more evident compared to implants, for PCoA (Principal Coordinates Analysis), and volatility analysis, regardless of the group. Analysis over time (core microbiome and heatmap) showed a higher number of bacteria at 21 days, suggesting maturation of the biofilm. Considering the limitations of the present study, it is possible to conclude that experimental gingivitis and mucositis, despite the absence of clinical differences, may present differences in inflammatory markers and microbiological parameters, with no significant effect of periodontal history on microbiological changes (AU) | |
| FAPESP's process: | 19/01522-7 - Clinical, immunological and microbiological evaluation of experimental Gingivitis and peri-implant Mucositis in patients with history of Agressive Periodontal Disease |
| Grantee: | Tamires Pereira Dutra |
| Support Opportunities: | Scholarships in Brazil - Doctorate |