Microbiological and chemical study of cultivable associated bacteria of the marine sponge Tedania brasiliensis

Author(s)

Jairo Iván Quintana Bulla

• Document type: Doctoral Thesis
• Press: São Carlos.
• Institution: Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT)
• Defense date: 2022-08-17

Examining board members

Roberto Gomes de Souza Berlinck; Patricia Machado Bueno Fernandes; Moacir Rossi Forim; Monica Tallarico Pupo; Thiago André Moura Veiga

• Advisor: Roberto Gomes de Souza Berlinck

Abstract

Marine sponges are recognized as an important source of biologically active compounds. Evidences accumulated over the last decades have proven that bacteria are the true producers of several sponge secondary metabolites, either symbionts or associated to these animals. Considering that bromopyrrole alkaloids related to pseudoceratidine previously isolated from the marine sponge Tedania brasiliensis are structurally closely related to brominated pyrroles isolated from marine bacteria, in this work, 87 cultivable bacterial strains isolated from T. brasiliensis were investigated to establish whether these strains were or not producers of pseudoceratidine derivatives. Results of dereplication analyses obtained for extracts of growth media of these bacteria in different growing conditions did not show the production of these compounds. Therefore, metabolomics and dereplication investigations were performed with the extracts of growth media produced by these bacteria. Use of an untargeted metabolomics approach employing modern computational tools like XCMS Online and GNPS under different culture conditions, combined with biological activity results, lead to the isolation and complete structural elucidation of four new glycoglycerolipids (J55_C8 to J55_C11) from the bacterial strain Microbacterium testaceum J55 grown in YEME medium. The approach employed and the results obtained demonstrated the utility of metabolomics tools at the early stages of the chemical and biological screening process, aiming to prioritize the selection of bacterial strains, as well as to direct the isolation and purification of secondary metabolites, therefore improving the efficiency of the discovery of potentially new bioactive metabolites. (AU)

• FAPESP's process: 16/16033-3 - Bromopyrrole alkaloids from the Cultured Microbiome of the Marine Sponge Tedania brasiliensis
• Grantee: Jairo Iván Quintana Bulla
• Support Opportunities: Scholarships in Brazil - Doctorate