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| Author(s): |
Cairo Monteiro de Oliveira
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2021-11-25 |
| Examining board members: |
Edison Luiz Durigon;
Erney Felicio Plessmann de Camargo;
Wilson Barros Luiz;
Enrique Mario Boccardo Pierulivo
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| Advisor: | Edison Luiz Durigon |
| Abstract | |
Arboviruses are viruses transmitted by arthropod vectors that predominate in tropical areas of the world. The brazilian territory taken by tropical forests and other natural ecosystems that allow ideal conditions for the presence of Arbovirus. A number of Arboviruses have already been detected and isolated throughout the territory with many still endemic such as Dengue virus (DENV), Zika virus (ZKV), Chikungunya virus (CHIKV) and many others circulating in nature through reservoir hosts and arthropod vectors such as dipterans, ticks, etc. which often cause disease outbreaks in animals and humans. In this context, the states of Rondônia and Amazonas, which owns part of its territories covered by amazon forest have an ecosystem that favors the existence of different species of arthropod vectors that together with anthropogenic actions and poor basic sanitation conditions allow potential transmission and establishment of arboviruses in these regions. Objective: The present work aims to monitor the presence of Arboviruses of medical importance such as Flavivirus, Alphavirus and Bunyavirus in species of dipterans collected in the states of Rondônia and Amazonas, Brazil. Methods: In this study, three RT-qPCR (Reverse Transcrption Quantitative Polymerase Chain Reaction) assays with Sybr Green were standardizes fot detection of Arboviruses (Flavivirus, Alphavirus and Bunyavirus). In addition, diptera species were captured in different regions throughout the Amazonas and Rondônia states between 2017-2019, and after a process of maceration and extraction nucleic acids were subjected to arbovirus research using standardized protocols. Results: The assays proved to be effective for researching interest arbovirus. To date, of the 9,299 dipterans collected approximately 6,798 dipterans have been processed and analyzed of which: 69.46% (n=4714) belong to the family Culicidae, 15.5% (n=1050) to Ceratopogonidae, 8.2% (n=555) to Psychodidae, 6.8% (462) to Tabanidae and 0.04% (n=3) to Simullidae divided into more than 35 distinct species. Flavivirus, Alphavirus or Bunyavirus were not detected from the total of the analyzed dipterans. Conclusion: Despite the good efficiency of the protocols there was no detection arbovirus in the analyzed mosquito pools. This work provides important data regarding the current Brazilian epidemiological situation especially in the possible \"spot\" of transmission and dissemination of these viruses without discarding the potential circulation in the regions studied. (AU) | |
| FAPESP's process: | 18/18867-4 - Monitoring and molecular characterization of arboviros isolated from dippers of the Brazilian west Amazon (State of Rondônia) |
| Grantee: | Cairo Monteiro de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Master |