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| Author(s): |
Eloize Cristina Chiarreotto Ropelle
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | Limeira, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas |
| Defense date: | 2018-03-15 |
| Examining board members: |
José Rodrigo Pauli;
Angélica Sartori Cintra;
Adelino Ramos Sanches da Silva;
Ellen Cristini de Freitas;
Leandro Pereira de Moura
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| Advisor: | José Rodrigo Pauli |
| Abstract | |
Physical exercise is considered an effective non-pharmacological strategy to prevent or treat type 2 diabetes. However, the effects of exercise in skeletal muscle and in the glucose homeostasis are not fully understood. Here, we investigate the role of acute exercise on insulin sensitivity in the skeletal muscle of obese mice, highlighting the anti-inflammatory effects of IL-10. Male C57BL/6j and IL-10 knockout mice were fed on high-fat diet and then submitted to acute exercise protocol. We monitored the glucose profile and insulin resistance markers in the skeletal muscle. We also performed the bioinformatics analysis from skeletal muscle of strains of genetically-diverse BXD mice. We demonstrated that acute exercise improved the insulin tolerance in obese mice. This data was accompanied by the reduction of JNK and IKK phosphorylation in the gastrocnemius muscle. Conversely, exercise increased IL-10 protein levels in the gastrocnemius muscle. Bioinformatics analysis revealed a negative correlation between Il-10 mRNA levels and body weight, epidydimal fat pad weight and several inflammatory markers in the skeletal muscle of obese BXD mice. Finally, acute exercise failed to reduce IKK phosphorylation in the muscle of obese IL-10 knockout mice. These data demonstrate that the anti-inflammatory effect of acute exercise in muscle requires, at least in part, the IL-10 action to suppress IKK activity in the skeletal muscle and improves insulin sensitivity in mice (AU) | |
| FAPESP's process: | 13/23723-8 - Anti-inflammatory effect of exercise on insulin sensitivity in skeletal muscle of obese rodents: role of interleukin 10 |
| Grantee: | Eloize Cristina Chiarreotto Ropelle |
| Support Opportunities: | Scholarships in Brazil - Doctorate |