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| Author(s): |
Marlus da Silva Pedrosa
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) |
| Defense date: | 2022-02-04 |
| Examining board members: |
Igor Studart Medeiros;
Alyne Simoes Gonçalves;
Jose Nicolau
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| Advisor: | Fernando Neves Nogueira |
| Abstract | |
The aim of this study was to verify the effect of diabetes in the presence or absence of insulin on the expression, quantity and activity of key enzymes of glycolytic metabolism, glycogen, Krebs cycle and lipid metabolism in the submandibular (SM) glands of diabetic rats induced by streptozotocin. Twenty-four male Wistar rats were divided into three groups (n = 8 in each): control (C), diabetic (D), and diabetic treated with insulin (10 U/kg) (D+I). In the SM glands, the mRNA levels and activity of of hexokinase (HK), phosphofructokinase 1 (PFK-1), pyruvate kinase (PK), lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G6PD), pyruvate dehydrogenase (PDH), acetyl-CoA synthase (ACS), citrate synthase (CS), succinil-CoA sintetase (SUCLA), alpha-ketoglutarate dehydrogenase (-KGDH), succinate dehydrogenase (SDH), carinitine palmitoil transferase 1 (CPT-1), acetil-CoA carboxilase (ACC), atp-citrate liase (ACLY), acetyl-CoA dehydrogenase (ACAD), glycogen synthase (GS), and glycogen phosphorylase (GP) were analyzed. The quantification of enzymes was determined in cases of discrepancy between gene expression and enzymatic activity. The results showed that the hyperglycemic state in diabetes altered the expression and activity of enzymes involved in the energetic metabolism of the SM glands leading to novo fatty-acid synthesis and consumption of glycogen and acetate. The insulin administration increased the pentose phosphate pathway and lipid synthesis and degradation in the SM gland. Altogether, the data suggest that metabolic alterations present in the SM gland in DM are mainly insulin-dependent and reversible. (AU) | |
| FAPESP's process: | 20/07903-0 - Effect of Diabetes and insulin on the production and activity of key enzymes of energetic metabolism of the submandibular gland of rats |
| Grantee: | Marlus da Silva Pedrosa |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |