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| Author(s): |
Bianca Tavares Rangel
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Bauru. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) |
| Defense date: | 2024-06-12 |
| Examining board members: |
Karin Hermana Neppelenbroek;
André Ulisses Dantas Batista;
Vinícius Carvalho Porto;
Joel Ferreira Santiago Junior
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| Advisor: | Karin Hermana Neppelenbroek |
| Abstract | |
The objective of this study was to synthesize and characterize polymethylmethacrylate (PMMA) cerium (Ce) nanofibers and investigate the effect of their incorporation on the biological, physical-mechanical properties and cytotoxicity of PMMA acrylic resin for removable denture base. PMMA-Ce nanofibers with different concentrations of cerium (5%, 10%, 15% and 20%) were produced using the solution blow spinning (SBS) technique and characterized using scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR), thermogravimetry, differential scanning calorimetry and inductively coupled plasma optical emission spectroscopy. Furthermore, they were evaluated for antifungal activity using the agar diffusion test. Different proportions (0.5%, 1%, 2.5% and 5% by weight) of PMMA-Ce nanofibers were incorporated into a heat-polymerized acrylic resin (Onda-Cryl). The material modified by nanofibers was evaluated for antifungal activity, flexural strength, Knoop microhardness, surface roughness (Sa and Sq), contact angle, color parameters (L*, a* and b*), and cytotoxicity. Data were analyzed using the Kruskal-Wallis and Dwass-Steel-Chritchlow-Fligner tests (agar diffusion, CFU/mL counts, hardness, surface roughness and color parameter L*), 1-way ANOVA and Tukey\'s test (flexural strength, hardness, contact angle, and color parameters a* and b*) and repeated measures ANOVA and Tukey test (cytotoxicity). The 15% PMMA-Ce nanofibers showed antifungal activity (p<0.05) and released Ce ions for 14 days. After incorporation of 15% nanofibers, the acrylic resin showed no antifungal activity in agar diffusion test and CFU/mL counts in the adhesion phase and biofilm formation of Candida albicans (p>0.05), which was confirmed by release of Ce ions below detectable limits. In proportions of 2.5% and 5%, the nanofibers resulted in reduction in flexural strength and change in color of the acrylic resin (p<0.05). There was a decrease in the hardness of the modified material in proportions of 0.5% and 5% (p<0.05). Regardless of the proportion tested, the nanofibers resulted in an increase in the contact angle of the acrylic resin (p<0.05), without changing its surface roughness (p>0.05). Regarding cytotoxicity for HGF-1 and NOK-SI cells, PMMA modified by different proportions of nanofibers showed no significant difference from pure PMMA in the time intervals analyzed (p>0.05). The incorporation of PMMA-Ce nanofibers into PMMA acrylic resin resulted in no antifungal activity and had a deleterious effect on the physical-mechanical properties of the modified material, without altering its cytotoxicity. (AU) | |
| FAPESP's process: | 22/01859-4 - Incorporation of polymethylmethacrylate (PMMA) - cerium (Ce) nanofibers in acrylic resin for dental prostheses: evaluation of physical-mechanical properties, biocompatibility and antifungal activity against Candida albicans |
| Grantee: | Bianca Tavares Rangel |
| Support Opportunities: | Scholarships in Brazil - Master |