Full text
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| Author(s): |
Carolina Bosso André
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2013-02-19 |
| Examining board members: |
Marcelo Giannini;
Caio Cezar Randi Ferraz;
Evelise Machado de Souza
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| Advisor: | Marcelo Giannini |
| Abstract | |
The evolution of adhesive systems have produced significant improvement in marginal sealing ability and durability of dental restorations. Besides the monomers, solvents and initiators found in the adhesive systems, components such as, fluorides, fillers and antibacterial agents may also be found however there is still a lack of studies about their role. This study evaluated the morphology, the dentin bond strength and the antibacterial activity of three different adhesive systems (Gluma 2Bond, Clearfil SE Protect and Peak Universal Bond). Human coronal, occlusal dentin was used for bonding and the adhesives were used according to the manufacturer's instructions. A composite block was incrementaly built-up on dentin after adhesive application. Afterwards, teeth were sectioned for obtaining microtensile specimens. For resin-dentin bonded morphology, the teeth were prepared similarly to the bond strength test methodology. However, teeth were sectioned in only one direction (mesio-distal) to analyze the dentin-resin interface. Microtensile Bond strength test was performed after 24 hours or 1 year after storage in artificial saliva at 37oC. Microtensile data were analyzed by 2-way ANOVA and Tukey test. All adhesives formed hybrid layer and resin tags, which varied in thickness and number, respectively, according to the type of material used. In the antimicrobial activity test, 4 facultative bacteria were used: Staphylococcus aureus, Enterococcus faecalis, Lactobacillus casei and Streptococcus mutans. Inhibition Halo and Minimum Bactericidal Concentration were performed for all the bacterial samples. For halo inhibition data were xvi analyzed by Dunn and Kruskal Wallis tests. There was no statistical difference in bond strength between the Gluma 2Bond and Peak Universal Bond adhesives. The Clearfil SE Protect showed the lowest bond strength to dentin. Adhesives showed no reduction in bond strength after one year of storage in human saliva. In the evaluation of the antimicrobial activity only Gluma 2Bond showed no inhibition halo, but was bactericidal after 24 hours of contact with the inoculums for all bacteria tested. The Cleafil SE Protect presented inhibition halo, as well as his primer, and was bactericidal against all bacterial strains tested at specific times of contact with the inoculums. The adhesive Peak Universal Bond showed inhibition halo, when cured, to only two bacteria, and was not bactericidal at any of the times, for all tested bacteria. Results suggested that the self-etching adhesive showed the lowest bond strength mean compared to Peak Universal Bond, however the dentin bond strength of all the adhesive systems remained stable after the storage for one year. The phosphoric acid caused the formation of a thicker hybrid layer and higher amount of resin tags when compared to the self-etching adhesives. Gluma 2Bond and Clearfil SE Protect exhibit bactericidal potential. The Clearfil SE Protect and Peak Universal Bond also demonstrated inhibitory potential (AU) | |
| FAPESP's process: | 10/13599-0 - Evaluation of resin-dentin bond strenght and antimicrobial activity of adhesive systems. |
| Grantee: | Carolina Bosso André |
| Support Opportunities: | Scholarships in Brazil - Master |