Full text
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| Author(s): |
Karla Evelyn Cook
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Campinas, SP. |
| Institution: | Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba |
| Defense date: | 2013-02-28 |
| Examining board members: |
Cinthia Pereira Machado Tabchoury;
Carolina Patrícia Aires;
Wander José da Silva
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| Advisor: | Cinthia Pereira Machado Tabchoury |
| Abstract | |
Byproducts of starch hydrolysis, with different dextrose equivalent (DE) numbers, which is a measure of its reducing power, have been widely employed by food industry due to their properties. Even though starch is considered non or slightly cariogenic, starch hydrolysates are able to lead to a substantial drop in the biofilm pH, suggesting a cariogenic potential. However, no study compared the effect of starch hydrolysates with different DEs on dental demineralization. Thus, the objective of this work was to evaluate the effect of starch hydrolysates with different DEs on enamel and dentin demineralization in situ, as well as the acidogenicity of dental biofilm formed in vivo and in situ. The in vivo study had a crossover design, with 6 experimental phases and 12 volunteers. The volunteers refrained from oral hygiene of the posterior teeth for 3 days, while rinsed 5 x/day with one of the following treatments: purified water (negative control); sucrose , glucose, starch hydrolysate DE 5, starch hydrolysate DE 20, starch hydrolysate DE 40. The solutions were prepared at 20%. On the 4th day of each phase, the pH of the biofilm formed in vivo was evaluated at time zero (baseline) and after 5, 15, 30 and 60 min of the rinsing with the respective treatment solution. The in situ experiment had a crossover and split-mouth design, regarding the treatments, and was carried out in 3 experimental phases of 14 days. Fourteen volunteers wore a palatal device containing 8 dental slabs, 2 enamel and 2 dentine slabs on each side of the device. The volunteers were submitted to the following treatments: purified water (negative control), 2% starch, 10% sucrose, 10% starch hydrolysate DE 5, 10% starch hydrolysate DE 20 and 10% starch hydrolysate DE 40. The solutions were extra-orally dripped over the slabs 8 x/day. On the 15th day, the biofilm acidogenicity was measured (baseline and 5 min after exposure to the respective solutions of that phase) and the dental slabs were collected for determination of surface and cross-sectional hardness. In the in vivo experiment, all treatments, except water, caused significant pH drops 5 min after rinsing, with no statistical difference among the starch hydrolysates. At 30 min, no statistically significant differences were found among the carbohydrate-containing treatments, which reached baseline pH values at 60 min. In the in situ experiment, all starch hydrolysates lowered the biofilm pH. Regarding mineral loss, the starch hydrolysates DEs 20 and 40 caused significantly higher losses than water and starch, either to enamel or dentine, however lower than sucrose. In conclusion, the starch hydrolysates are capable of inducing enamel and dentine demineralization, which depends on their dextrose equivalent (AU) | |
| FAPESP's process: | 09/13149-7 - Effect of starch hydrolysates with different dextrose equivalent on biofilm acidogenicity and dental demineralization |
| Grantee: | Karla Evelyn Cook |
| Support Opportunities: | Scholarships in Brazil - Master |