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| Author(s): |
Tábata Leal Nascimento
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-12-13 |
| Examining board members: |
Elen Haruka Miyabara;
Marcelo Saldanha Aoki;
Sandro Massao Hirabara
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| Advisor: | Elen Haruka Miyabara |
| Abstract | |
Heat shock proteins play a key regulatory role in cellular defense. In order to investigate the role of the inducible 70-kDa heat shock protein (HSP70) in skeletal muscle atrophy and subsequent recovery, extensor digitorum longus and soleus muscles from overexpressing HSP70 transgenic mice were immobilized during 7 days and subsequently released from immobilization and evaluated after 7 days. There was a decrease in myofiber cross-sectional area after immobilization in both wild type and HSP70 mice, but only myofibers from HSP70 mice recovered their size. The number of satellite cells and the muscle tetanic contraction were unchanged only in the muscles from HSP70 mice. In addition, the increase of atrogin-1 and MuRF-1 gene expression was attenuated in HSP70 mice. Therefore, our study suggests that the HSP70 is important for structural and functional recovery of muscles after immobilization and this effect might be associated with preservation of satellite cell amount and regulation of atrogenes. (AU) | |
| FAPESP's process: | 10/04813-8 - Role of inducible heat shock protein 70 (HSP70i) in muscle atrophy and subsequent recovery. |
| Grantee: | Tábata Leal Nascimento |
| Support Opportunities: | Scholarships in Brazil - Master |