Gene expression analysis after interaction between Aggregatibacter actinomycetemcomitans and epithelial cell.

Author(s)

Josely Emiko Umeda

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2010-11-17

Examining board members

Marcia Pinto Alves Mayer; Regina Lúcia Baldini; Gustavo Pompermaier Garlet; Denise Madalena Palomari Spolidorio; Carlos Pelleschi Taborda

• Advisor: Marcia Pinto Alves Mayer

Abstract

Aggregatibacter actinomycetemcomitans (A.a) is associated with the etiology of aggressive periodontitis. The activation of signaling pathways by the host and bacterial cells occurs during infection. Objectives: to determine the transcription of genes related to bacterial virulence and epithelial cell after bacterial-epithelial cell interaction. Methods: A.a strains were co-cultured with epithelial cells OBA-9. The transcription of A.a virulence genes and genes belonging to signaling transduction pathway were determined by RT-qPCR and the concentration of cytokines was determined by ELISA. Results: the transcription of some virulence genes is strain and time specific. Fifteen genes were up-regulated in OBA-9 cells. High levels of GM-CSF, TNF-<font face=\"Symbol\">&#945 and ICAM-1 were detected in infected OBA-9 cells and tissues of patients with periodontitis. Conclusion: the interaction between A.a and epithelial cell can modulate the host response with induction of factors which exacerbate inflammatory process. (AU)