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| Author(s): |
Karinna Chouman
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-02-01 |
| Examining board members: |
Luiziana Ferreira da Silva;
Flavio Alterthum;
Regina Lúcia Baldini
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| Advisor: | Luiziana Ferreira da Silva |
| Abstract | |
Polyhydroxyalkanoates (PHA) are natural polyesters accumulated by many bacteria under nutritional stress conditions; with the role of as carbon and energy sources. In Brazil, the sugarcane bagasse is an important lignocellulosic crop that can be used as precursors for other products, for instance, PHAs. E. coli are capable to select, in a mixture of sources of carbon, that promotes the best bacterial growth rate. This phenomenon is known as catabolite repression (CR). The aim of this work is to create strategies to reduce the repression catabolite process. During this project, sugar mixtures have been simultaneously consumed. Genes were inserted in E. coli VH33 and the wild type W3110. The M9 medium without supplementation was selected and used to accumulate bioreactor assay. The mutant strain VH33 pSK-::phaCABCn accumulated up to 27% of PHA. In the metabolic flux analysis, it was observed that LFM 862 strain was already in the theoretical maximum capacity. The catabolic repression was practically abolished in this assay. Bioreactor assays were made using sugarcane hydrolysate, but the strain was not capable to grow. (AU) | |
| FAPESP's process: | 10/02165-9 - Production of biodegradable polymers by recombinant Escherichia coli from sugarcane bagasse hydrolysate |
| Grantee: | Karinna Chouman |
| Support Opportunities: | Scholarships in Brazil - Master |