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| Author(s): |
Érica de Almeida Duque
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-03-22 |
| Examining board members: |
Carolina Demarchi Munhoz;
Daniel Carneiro Carrettiero;
Jean Pierre Schatzmann Peron
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| Advisor: | Carolina Demarchi Munhoz |
| Abstract | |
Although glucocorticoids (GCs) are the most commonly prescribed anti-inflammatory worldwide, high levels of GCs enhance some aspects of the inflammatory response in brain regions of rats, dependent on activation of GR. In this study, we aim to evaluate the effects of glucocorticoids in some aspects of the inflammatory response LPS-induced in populations of cells (neurons, astrocytes, and microglia) in primary frontal cortex derived from newborn rats. Through EMSA and immunofluorescence assays, we evaluated the indicative factor NFKB activation in mixed and enriched cultures exposed to pretreatment with CORT, followed by the LPS inflammatory stimulus. We found that CORT did not exerted its classic anti-inflammatory activity, whereas it was not able to decrease the activation of NFKB LPS-induced in mixed cultures, suggesting be partially dependent on the GR activation, GCs concentration and cellular interactions, since astrocytes in mixed cultures exhibit different responses relative to NFKB, but neurons and microglia did not. (AU) | |
| FAPESP's process: | 10/13932-0 - Glucocorticoid effects on LPS-induced inflammation in rat primary frontal cortex cultures |
| Grantee: | Erica de Almeida Duque |
| Support Opportunities: | Scholarships in Brazil - Master |