Oophorectomy high fat diet and DHEA: effects on insulin sensitivity and insulin secretion on isolated islets rats.

Author(s)

Katherine Maria de Araujo Véras

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-07-15

Examining board members

Carla Roberta de Oliveira Carvalho; Luciana Chagas Caperuto; Angelo Rafael Carpinelli

• Advisor: Carla Roberta de Oliveira Carvalho

Abstract

Natural and induced privation of sexual hormones contributes to the development of several metabolic and endocrine disorders. The present study evaluated if DHEA supplementation, the most abundant steroid in humans, would improve the insulin sensitivity and secretion as well as the glucose tolerance, in high fat diet fed ovariectomized rats (OHL). Ovariectomy (OVX) reduced the physiological female protection against the weight gain. Although no effect upon adipose depot-specific action of DHEA has been found, DHEA has corrected the blood insulin levels and HOMA IR. In addition, DHEA has improved peripheral insulin action by the glucose disappearance rate, kitt. The islets area was increased in all ovariectomized groups. Pancreatic islets from DHEA-treated rats showed an increased in the Akt serine phosphorylation status and restored glucose-stimulated insulin secretion. Our results suggest that DHEA can promote protective effects by increasing the insulin sensitivity in females castrated rats exposed to health risk factors. (AU)

• FAPESP's process: 09/04009-7 - Effect of DHEA treatment on insulin synthesis and secretion in oophorectomyzed rats
• Grantee: Katherine Maria de Araújo Veras
• Support Opportunities: Scholarships in Brazil - Master