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High-fat diet and aging impair insulin sensibility and intracellular insulin signaling proteins in hypothalamus of female mice.

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Gabriela Virginia Moreira
Total Authors: 1
Document type: Master's Dissertation
Press: São Paulo.
Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
Defense date:
Examining board members:
Carla Roberta de Oliveira Carvalho; Eliana Hiromi Akamine; Marco Antonio de Carvalho Filho
Advisor: Carla Roberta de Oliveira Carvalho

In males, aging and obesity induced by high-fat diet (HFD) show defects in insulin action, but there is a lack of information about females. We evaluated the insulin sensitivity, glucose tolerance, estrous cycle and proteins expression of the intracellular insulin pathway in the hypothalamus and skeletal muscle of female C57BL6. Ten month-old mice and 4 mo HFD fed mice had increased fat pads, insulin resistance and glucose intolerance. The HFD fed group showed impaired estrous cycle pattern followed by reduced number of corpora lutea. There were also, increased IR and PI3K protein levels in the hypothalamus and increased PI3K/AKT1 and IL-6 proteins in skeletal muscle. In hypothalamus of 10 month-old mice there was a decreased IR and TNF alpha proteins level. Thus, our data confirm the association between obesity, insulin resistance and glucose intolerance induced by HFD and aging in female mice. Moreover, there were distinct change proteins in expression of the hypothalamic IR in both animal models. (AU)

FAPESP's process: 09/11986-9 - Effect of hyperlipidic diet on the expression of related proteins via intracellular insulin in hypothalamus of female mice at the stages of diestrus and proestrus.
Grantee:Gabriela Virginia Moreira
Support Opportunities: Scholarships in Brazil - Master