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| Author(s): |
Felipe Alves Morais
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-11-11 |
| Examining board members: |
Viviane Fongaro Botosso;
Antonio Walter Ferreira;
Luiz Tadeu Moraes Figueiredo;
José Antonio Jerez;
Ronaldo Zucatelli Mendonça
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| Advisor: | Edison Luiz Durigon |
| Abstract | |
The genus Hantavirus of the family Bunyaviridae includes a large number of rodent-borne viruses that are distributed worldwide. The occurrence is due mainly to ecological disturbances and it is transmitted to the humans through inhalation of virus particles contained in the excreta of wild rodents. Two different human diseases known to be caused by Hantavirus: are Hemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Cardiopulmonary Syndrome (HPS). The main objective of this study was detected antibody against hanatavirus (IgG) by ELISA, in Amazon region and Brazilian Southwest populations who live in contact with the wild rodents, using recombinant protein (antigen) of the Araraquara virus expressed in Escherichia coli. We study 1308 human sera (1078 from Amazon region) and there were found 59 (5%) positive sera. From the city of Machadinho do Oeste RO (2003 year), 633 sera were analysed, where there were found to be 20 positive (4.5%) serums. In Machado river RO (2005 year), 435 sera of the river-dwelling population were analysed where there were found 39 (5%) positive sera, respectively. After analysis was accomplished for 151 human sera coming from the Vale do Ribeira - SP, in 2007, and 84 from the Pontal do Paranapanema - SP, in 2008, 14 (9%) and 6 (7%) of the samples were observed to be positive, respectively. (AU) | |
| FAPESP's process: | 05/01603-4 - Serological detection of hantavirus in wild and human animal samples in the state of São Paulo by Western Blotting and ELISA assay using recombinant protein developed in Brazil |
| Grantee: | Felipe Alves Morais |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |