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| Author(s): |
Flávio Augusto Cardozo
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-08-30 |
| Examining board members: |
Irma Nelly Gutierrez Rivera;
Rubens Mendes Lopes;
Elisabete Jose Vicente
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| Advisor: | Irma Nelly Gutierrez Rivera |
| Abstract | |
Chitinolytic bacteria have key role in nutrient cycling through the chitin degradation in the marine ecosystem. The hydrolysis of chitin is performed by chitinases, which can be used in biotechnological processes. The study aimed to evaluate the optimal conditions for cultivation of selected chitinolytic bacteria of the Aeromonas genus and the degradation of chitin during 96 hours of culture. Moreover, characterize them by 16S rRNA gene full sequencing and MLSA. The study showed that the isolates represent two species of the Aeromonas genus. The isolates had different behavior in relation under conditions pre-determined. The halos of chitin hydrolysis were not related to enzymatic activity or to their products of chitin hydrolysis quantified. The hightest activities of chitobiosidases and endochitinases were observed in 24 hours and the N-acetyl-glicosaminidases in 96 hours. The enzyme profiles differ between isolates and during 96 hours of cultivation showing diversity enzyme. (AU) | |
| FAPESP's process: | 10/13004-6 - Chitinolytic activity evaluation by Aeromonas genus strains isolated from the marine ecosystem. |
| Grantee: | Flávio Augusto Cardozo |
| Support Opportunities: | Scholarships in Brazil - Master |