Full text
| |
| Author(s): |
Carlos Alexandre Carollo
Total Authors: 1
|
| Document type: | Doctoral Thesis |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2008-03-28 |
| Examining board members: |
Dioneia Camilo Rodrigues de Oliveira;
Ian Castro Gamboa;
Massuo Jorge Kato;
Monica Tallarico Pupo;
João Maximo de Siqueira
|
| Advisor: | Dioneia Camilo Rodrigues de Oliveira |
| Abstract | |
Mikania is the largest gender of the tribe Eupatorieae, with about 430 species, being distributed thoroughly in Brazil, with approximately 200 described species. In this gender, Mikania cordifolia, M. micrantha and M. glomerata are commonly called \"guaco\" and are among the more popularly used species, mainly for the treatment of fever, rheumatism, diseases of the breathing tract and against snake bites. In the present work, phytochemical studies of these three species were carried out and the effects of the fertilization types, the solar radiation and the hydric stress upon the production of secondary metabolites were analyzed. Firstly, through phytochemical studies, 62 compounds were identified. The polar fractions of the species presented as majority compounds phenylpropanoic derivatives: 3,5-Dicaffeoylquinic acid in M. glomerata and M. micrantha and Fertaric acid in M. cordifolia. In M. glomerata were also identified glucaric acid derivatives di- and tri-esterified with cafeic acids, which are no described in the literature until the moment, besides sulfated flavonoids, which are also present in M. micrantha. In the apolar fractions the triterpenes presence were verified mainly in M. cordifolia and kaurenoic acid derivatives in M. glomerata and M. micrantha. The sesquiterpene lactones were found in M. cordifolia (melampolide skeleton) and in M. micrantha (micranolide skeleton). The analysis of M. glomerata and M. cordifolia cultivars revealed a great influence of the cultivation conditions in the secondary metabolites composition. The experiments showed a great variation among the concentration of the compounds in the two species of Mikania, being verified that the cultivation conditions and mainly the taxes of solar radiation are of extreme importance in the accumulation of these metabolites. The hydric stress didnt have significant effects in the accumulation of substances in M. glomerata. The histochemical analyses were effective in supply information regarding location of phenolic compounds in the leaves of M. glomerata. (AU) | |