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| Author(s): |
Gabriel Salles Barberio
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Bauru. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) |
| Defense date: | 2013-06-04 |
| Examining board members: |
Maria Aparecida de Andrade Moreira Machado;
Maria Ligia Gerdullo Pin;
Daniela Rios
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| Advisor: | Maria Aparecida de Andrade Moreira Machado |
| Abstract | |
This study brings a literature review about the current dengue status worldwide and in Brazil followed by a systematic review that highlights the dengue diagnosis through saliva. A prospective study was also made, which evaluated the dengue virus infection diagnosis accuracy through the NS1 antigen detection in saliva samples using ELISA assays. The NS1-ELISA results in saliva were compared to the IgM-ELISA and IgG-ELISA serology. A total of 44 saliva samples were obtained from November 2012 to February 2013. The results showed that de NS1-ELISA presented a sensibility of 0.73, specificity of 0.94, Positive Predictive Value of 0.95, Negative Predictive Value of 0.70, Positive Likelihood Ratio of 13.15, Negative Likelihood Ratio of 0.28. Having these findings in mind, it is possible to suggest that the NS1 detection in saliva may be an important diagnostic tool in special cases, such as people that fear needles, with blood dyscrasias, babies, children, as well as to quickly monitor epidemics and their dissemination. (AU) | |
| FAPESP's process: | 11/14882-0 - NS-1 biomarker identification in the saliva and the diagnosis of dengue fever |
| Grantee: | Gabriel Salles Barbério |
| Support Opportunities: | Scholarships in Brazil - Master |