Evaluation of healing of surgically created bone defects in rat calvaria treated with bovine grafts (Bio-Oss®) associated with low level laser. Histological and histometric study

Author(s)

Mércia Jussara da Silva Cunha

• Document type: Master's Dissertation
• Press: Bauru.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB)
• Defense date: 2013-03-04

Examining board members

Ana Lúcia Pompéia Fraga de Almeida; Carla Andreotti Damante; Sergio Luis Scombatti de Souza

• Advisor: Ana Lúcia Pompéia Fraga de Almeida

Abstract

This study histologically evaluated the effect of low level laser on the bone healing of critical size defects (CSD- 5mm) surgically created in rat calvaria, associated or not with inorganic bovine bone (Bio-Oss®). Sixty male rats (Rattus norvegicus, albinus, Wistar) were divided in 6 groups: C (control), L (low level laser - GaAlAs, 730nm, 100mW, 6J, 210J/cm2), AO (autogenous bone) OAL (autogenous bone + low level laser), BO (inorganic bovine bone), BOL (inorganic bovine bone + low level laser). The animals were killed after 30 days. The quantity of newly formed bone and area of remaining particles of the implanted materials were calculated as percentages of the original defect area. Data were statistically analyzed (Kruskal Wallis test, Dunn test; p<0.05). The groups irradiated with low level laser, L (47.67% ± 8.66%), OAL (39.15% ± 16.72%) and BOL (48.57% ± 28.22%) presented greater area of new bone formation than the groups C (9.96% ± 4.50%), OA (30.98% ± 16.59%) and BO (11.36% ± 7.89%), which were not irradiated. Also, they were significantly better than group C. within the limits of this study, it was concluded that the laser accelerated the new bone formation and resorption of particles of graft materials, thus it may be considered an interesting therapeutic option for association with biomaterials in reconstructive bone surgeries. (AU)

• FAPESP's process: 10/13170-3 - Analysis of healing of surgically created bone defects in rat calvaria treated with bovine grafts (Bio-Oss) associated with low level laser. Histological and histometric study
• Grantee: Mércia Jussara da Silva Cunha
• Support Opportunities: Scholarships in Brazil - Master