Effect of low-level laser and the protein purified from natural latex (Hevea brasiliensis) on crush injury of the sciatic nerve in rats: morphologic, immunohistochemical and ultrastructural study.

Author(s)

Fernando José Dias

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-02-20

Examining board members

Ii Sei Watanabe; Maria Lucia Zaidan Dagli; Valeria Paula Sassoli Fazan; Mamie Mizusaki Iyomasa; Silvia Lacchini

• Advisor: Ii Sei Watanabe

Abstract

This study evaluated the low intensity laser (LBI-15J/cm2, 780nm) and latex protein (P1, 0.1%) in the crush (15 kgf, axonotmesis) of the sciatic nerve in rats. Groups: GIcontrol; GIIexposed; GIII-injured; GIV-LBI; GV-P1; GVI-LBI and P1. After 4 and 8 weeks were performed morphological, morphometric, immunohistochemical (VEGF and NGF) and ultrastructural analysis. There was improvement with the proposed treatments. After 4 weeks has not been possible to choose the best treatment, after 8 weeks, the animals that received the P1 associated or not to LBI had more capillaries and more organized nerve fibers. After 4 weeks the expressions of NGF and VEGF were higher in GIII, GIV and GVI and after 8 weeks they were decreased. The characteristics of the injured groups were closer to control after 8 weeks, revealing time-dependence, even in this period P1 showed better recovery because the LBI did not achieve the same results, and the association of treatments did not enhanced this recovery, suggesting that the improvement observed in this case refers to P1. (AU)

• FAPESP's process: 10/12018-3 - Effect of low-level laser and the protein purified from natural latex (Hevea brasiliensis) on the crush injury of the sciatic nerve of rats: Morphological, immunohistochemical and ultrastructural study
• Grantee: Fernando José Dias
• Support Opportunities: Scholarships in Brazil - Doctorate