Evaluation of the mechanisms involved on the endothelium-dependent vasodilation in aorta of isoproterenol-treated mice.

Author(s)

Angelo Bernak de Oliveira

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-02-20

Examining board members

Luciana Venturini Rossoni; Eliana Hiromi Akamine; Luciano dos Santos Aggum Capettini

• Advisor: Luciana Venturini Rossoni

Abstract

The aim of this thesis was to investigate the mechanisms involved on the endothelium-dependent vasodilation focusing on the role of caveolae and the possible interaction between endothelial nitric synthase (eNOS)/ nitric oxide and neuronal (nNOS)/ hydrogen peroxide (H2O2) in aorta of ISO-treated mice. Aortic rings were mounted in an organ bath for measurement of isometric tension. The expression of proteins was evaluated using Western blot. The results demonstrated that ISO treatment: 1) did not change acetylcholine (ACh) or NO donor-induced relaxation; 2) increases the caveolae participation in ACh and calcium ionophore-induced relaxation and caveolin-1 protein expression, while did not change caveolin-3; 3) increases the constitutive NOS modulation to ACh-induced relaxation, mainly through nNOS; 4) increases the H2O2 involvement on the vasodilation-induced to ACh and 5) increases the antioxidant proteins. It is concluded that β-AR hyperactivation ISO active vasodilators compensatory mechanisms in response to ACh in the aortas of mice. (AU)

• FAPESP's process: 11/16017-4 - Evaluation of the mechanisms involved on the endothelium-dependent vasodilation in aorta of isoproterenol-treated mice
• Grantee: Angelo Bernak de Oliveira
• Support Opportunities: Scholarships in Brazil - Master