CV Lattes
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Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. Escola Paulista de Medicina (EPM) (Institutional affiliation from the last research proposal) Birthplace: Brazil
Bachelor's degree in Chemistry from the Federal University of São Carlos (1994), Master's degree (1998) and Ph. Medicine, Federal University of São Paulo (2012). He is currently an associate professor at the Department of Biophysics at Escola Paulista de Medicina, Federal University of São Paulo and Principal Researcher at the Center for Translational Science and Development of Biopharmaceuticals (CCD-FAPESP). He has experience in studying the structure-activity relationship of enzymes, mainly peptidases (proteases) and beta-lactamases, through enzymatic kinetics associated with protein chemistry and spectroscopic techniques (fluorescence and circular dichroism). It has used new protein engineering techniques through the bio-incorporation of non-canonical (non-natural) amino acids in protein structure-activity studies and has sought to develop proteins modified with these amino acids with special chemical characteristics (fluorescent, greater reactivity, possibility of modification through click-chemistry and etc) for biotechnological applications. (Source: Lattes Curriculum)
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We intend to use two specific cell surface tumor biomarkers, the prostate specific membrane antigen (PSMA) and the carcinoembryonic antigen (CEA), as targets for directing fluorescent probes, antitumor drugs, or therapeutic bacteria. For this, two previously described and characterized nanobodies will be used for targeting these cell surface biomarkers: the anti-PSMA nanobody called NB7 a…
For both Zika and Dengue viruses the NS2/NS3 protease, which is formed during replication process into the host cell, is essential for the processing of the viral polyprotein. The inhibition of this enzyme blocks the replication of the virus in the host cell. Therefore, inhibitors for these proteases are potential antiviral drugs.The main objective of this project is the development of in…
Prolyl oligopeptidase (POP; EC 3.4.21.26) is a serine-peptidase present in various types of cells and tissues. Some studies have shown that POP is highly expressed in GABAergic and cholinergic interneurons from the thalamus and cortex. A distinguish feature of POP is its determined substrate specificity: POP hydrolyses peptide bonds after proline residues and only in oligopeptides with le…
In the last 4 years we have focused our research in site-specific incorporation techniques of non-natural amino acids in proteins. For this purpose two main methodologies have been developed: -the first one is the introduction of tryptophan analogs (5-fluor-Trp, 5-hidroxi-Trp, 7-aza-Trp, 5-bromo-Trp and 5-nitro-Trp) in the place of the Trp residues, by using E. coli strains compatible wit…
(Only some records are available in English at this moment)
Atopic Dermatitis (AD) is a disease that affects approximately 7.3% of the Brazilian population aged 6 to 7 years and has a prevalence of about 1% to 3% of the adult global population. AD is characterized by three main symptoms: excessive skin scaling, pruritus, and erythema (redness). It is a chronic disease that tends to diminish over the individual's lifetime. However, in some cases, t…
Coronavirus is a family of viruses composed of a wide variety of species capable of infecting different animals. Among them, SARS-CoV-2 is the species responsible for the COVID-19 disease, which generated the entire global pandemic scenario we are currently experiencing. The spike (S) protein has been one of the main targets of the scientific community due to its essential role in viral i…
Prolyl Oligopeptidase (POP; EC3.4.21.26) is a serine peptidase present in various tissues and cell types. Some studies show that it is highly expressed in GABAergic and cholinergic interneurons of the thalamus and cortex. A distinguishable POP feature is the cleavage after proline residues in oligopeptides smaller than 3kDa. Recent studies have indicated that the major POP action is on in…
The main goal of the present project is to study the hinge movement that the peptidase, timet oligopeptidase (TOP) may undergo upon substrate or inhibitor binding. Fluorescence techniques will be used to study this conformational change in great detail. TOP constructs will be prepared equipped with two donor-donor fluorescent probes (Trp residues) or donor-acceptor probes (5-F Trp - p-nit…
Nitrosylation is currently considered an important reversible post-translational modification of Proteins. The dynamic of enzyme nitrosylation/denitrosylation is important because occurs in a time order below milliseconds, contrasting with that of enzyme catalysis (seconds). Furthermore, some enzymes acts as pivotal regulators of NO-dependent signal transduction pathways. The overall obje…
The main goal of the present project is to study the hinge movement that the peptidase, timet oligopeptidase (TOP) may undergo upon substrate or inhibitor binding. Fluorescence techniques will be used to study this conformational change in great detail. TOP constructs will be prepared equipped with two donor-donor fluorescent probes (Trp residues) or donor-acceptor probes (5-F Trp - p-nit…
| 1 / 1 | Ongoing grants |
| 7 / 5 | Completed research grants |
| 3 / 3 | Ongoing scholarships in Brazil |
| 10 / 3 | Completed scholarships in Brazil |
| 1 / 1 | Completed scholarships abroad |
| 23 / 14 | All research grants and scholarships |
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