Manoel de Arcisio Miranda Filho

• Abstract

  This project aims to design new cationic peptide sequences with selective action against cancer cells and bacteria. With a multidisciplinary approach, the present project proposal will allow, besides the determination of structural aspects of the studied peptides, to simultaneously obtain temporal and spatial resolution of the mechanisms involved in the action of cationic peptides in cell…

  Improvement and design of cathonic peptidic sequences with antitumoral and antibacterial action, AP.R

• Abstract

  This project has as main objective to obtain dynamic images of changes in intracellular pH (pHi) in microglia cells. Unlike other pHi measurement methods, the use of fluorescence microscopy will provide us with simultaneously spatial and temporal resolution of the events involved in maintaining the microglial pHi. Three majors will be studied: 1) the relationship between the composition o…

  OBSERVING THE INTRACELLULAR pH OF MICROGLIAL CELLS, AP.R

• Abstract

  The increasing resistance of bacteria to conventional antibiotics has stimulated efforts to obtain antimicrobial compounds with different mechanisms of action. Among these, it has been notable peptides like amphipathic ±-helix peptides that operate mostly permeabilizing the cell membrane of the pathogen causing him to death. It is believed that electrical factors exert dominant role in pe…

  Electrophysiology of antimicrobial peptides in planar lipid bilayers, AP.R

(Only some records are available in English at this moment)

• Abstract

  The endocannabinoid system plays a central role in physiological processes by modulating its action. This system comprises highly hydrophobic endocannabinoid ligands (eCBs), cannabinoid receptors (CB1 and CB2) and proteins involved in metabolic issues. Opposite to the canonical vision of the system, which associates the endocannabinoid action with classical receptors, experimental evidenc…

  Assessment of endocannabinoids' action on cell membrane fluidity and intracellular calcium imaging, BP.IC

• Abstract

  The bullfrog (Lithobates catesbeianus) is originally from North American, but was introduced in Brazil in 1935, and nowadays it is commonly created in commercial ranches. Studies on morphological characterization of the stages of development of the female reproductive tract showed great similarity with the development of the Xenopus laevis frog. In both cases, it was possible to classify …

  Validation of an alternative animal model for the development of a heterologous expression system of ion channels and membrane receptors, BP.DR

• Abstract

  The voltage gated proton channel (HV1) plays an important role during microbial killing by phagocytes, such as neutrophils and microglia. Besides its physiological functions, recent studies have shown that HV1 channel participates in pathological states, not only increasing tissue damage in ischemic stroke, but also promoting the proliferation and survival of tumoral cells. Even though th…

  Molecular Analysis of Intracellular Trafficking of the Voltage Gated Proton Channel by Fluorescence Microscopy, BP.DR

(Only some records are available in English at this moment)