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Electrophysiology of antimicrobial peptides in planar lipid bilayers

Grant number: 12/02065-0
Support type:Regular Research Grants
Duration: August 01, 2012 - July 31, 2014
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal Investigator:Manoel de Arcisio Miranda Filho
Grantee:Manoel de Arcisio Miranda Filho
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The increasing resistance of bacteria to conventional antibiotics has stimulated efforts to obtain antimicrobial compounds with different mechanisms of action. Among these, it has been notable peptides like amphipathic ±-helix peptides that operate mostly permeabilizing the cell membrane of the pathogen causing him to death. It is believed that electrical factors exert dominant role in peptide-lipid interactions, resulting in the permeabilization of the membrane. Planar lipid bilayers are an excellent experimental model to study the action of peptides as mimicking the physicochemical aspects essential to biomembranes. In this project, our aim is determining the consequences of variation in lipid composition of the membrane to the action of antimicrobial peptides. These peptides interact with phospholipid membranes regardless of specific receptors, in a timely manner, and concentration dependent. The study will be carried out single-channel activity and membrane capacitance measurements. Our main goal is to understand the physicochemical aspects of biomembranes that affect the binding and pore activity of these peptides. Furthermore, structural factors of cholesterol molecule that determine the cell selectivity of these peptides will be investigated. This is expected to achieve a more realistic structure and pore-forming kinetics of antimicrobial peptides. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COSTA PETRIN, THAIS H.; FADEL, VALMIR; MARTINS, DANUBIA B.; DIAS, SUSANA A.; CRUZ, ANA; SERGIO, LUCIANA MARCIANO; ARCISIO-MIRANDA, MANOEL; CASTANHO, MIGUEL A. R. B.; DOS SANTOS CABRERA, MARCIA P. Synthesis and Characterization of Peptide-Chitosan Conjugates (PepChis) with Lipid Bilayer Affinity and Antibacterial Activity. Biomacromolecules, v. 20, n. 7, p. 2743-2753, JUL 2019. Web of Science Citations: 0.
BRITO DA SILVA, ANNIELLE MENDES; SILVA-GONCALVES, LAIZ COSTA; OLIVEIRA, FERNANDO AUGUSTO; ARCISIO-MIRANDA, MANOEL. Pro-necrotic Activity of Cationic Mastoparan Peptides in Human Glioblastoma Multiforme Cells Via Membranolytic Action. Molecular Neurobiology, v. 55, n. 7, p. 5490-5504, JUL 2018. Web of Science Citations: 3.
MARTINS, DANUBIA BATISTA; NASARIO, FABIO DOMINGUES; SILVA-GONCALVES, LAIZ COSTA; DE OLIVEIRA TIERA, VERA APARECIDA; ARCISIO-MIRANDA, MANOEL; TIERA, MARCIO JOSE; DOS SANTOS CABRERA, MARCIA PEREZ. Chitosan derivatives targeting lipid bilayers: Synthesis, biological activity and interaction with model membranes. Carbohydrate Polymers, v. 181, p. 1213-1223, FEB 1 2018. Web of Science Citations: 6.
MEDEIROS, DJALMA; SILVA-GONCALVES, LAIZ DA COSTA; BRITO DA SILVA, ANNIELLE MENDES; DOS SANTOS CABRERA, MARCIA PEREZ; ARCISIO-MIRANDA, MANOEL. Membrane-mediated action of the endocannabinoid anandamide on membrane proteins: implications for understanding the receptor-independent mechanism. SCIENTIFIC REPORTS, v. 7, JAN 27 2017. Web of Science Citations: 2.
SILVA, DEBORA E. S.; CALI, MARIANA P.; PAZIN, WALLANCE M.; CARLOS-LIMA, ESTEVAO; SALLES TREVISAN, MARIA TERESA; VENANCIO, TIAGO; ARCISIO-MIRANDA, MANOEL; ITO, AMANDO S.; CARLOS, ROSE M. Luminescent Ru(II) Phenanthroline Complexes as a Probe for Real-Time Imaging of A beta Self-Aggregation and Therapeutic Applications in Alzheimer's Disease. Journal of Medicinal Chemistry, v. 59, n. 19, p. 9215-9227, OCT 13 2016. Web of Science Citations: 7.

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