Carlos Augusto Pereira
CV Lattes
Secretaria da Saúde (São Paulo - Estado). Instituto Butantan (Institutional affiliation for the last research proposal) Birthplace: Brazil
Graduated in Biological Sciences at the "Universidade do Estado do Rio de Janeiro" (1970). PhD in Viral Immunology at "Universidade Estadual de Campinas" (1974). Invited Professor at the "Université de Strasbourg", France (1999-2006). Currently research investigator "1A senior" of the CNPq at the "Laboratório de Imunologia Viral do Instituto Butantan" ("PqC VI"). Experience in the areas of Biotechnology, Virology and Imunology. (Source: Lattes Curriculum)
- Program for prof. Michael Butler visit: training on glycosylation on recombinant protein, AV.EXT
Abstract
The aim of Prof. Michael Butler visit is to train researchers from Instituto Butantan (LIV) and Unifesp (ICT, Campus São José dos Campos) on Post-translational modifications (MPT) of recombinant proteins, focusing more specifically on Glycosylation, which has enormous implications over the quality of these molecules. Since, part of the program includes a gradute course, this training p...
- Study of the quantitative dinamics of heterologous gene transcription in transfected animals cells for bioprocess optimization, AP.R
Abstract
The quantitative RT-PCR (qRT-PCR) is an important tool for gene quantification and few studies have focused its use to study, monitor and control of heterologous gene expression dynamics in eukaryotic systems used for recombinant protein production. Most of the studies, in this area, use the qRT-PCR just as an indirect method for evaluating gene expression. Our aim, on the other hand, i...
- Development of a bioprocess with S2 cells for production and purification of the recombinant rabies virus glycoprotein and study of its antigenicity, AP.R
Abstract
The aim of the present research project is to promote a collaborative work involving Laboratories of the Instituto Butantan and the Escola Politecnica of the University of São Paulo to study and establish a bioprocess for the synthesis and extraction of the recombinant rabies virus glycoprotein (GPV) with high productivity and immunologic quality from S2 Drosophila melanogaster cells cu...
(Only some records are available in English at this moment)
| 24 / 8 | Completed research grants |
| 17 / 0 | Completed scholarships in Brazil |
| 3 / 0 | Completed scholarships abroad |
| 44 / 8 | All research grants and scholarships |
| Associated processes | |
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
| Publications | 12 |
| Citations | 115 |
| Cit./Article | 9.6 |
| Data from Web of Science | |
FABIANA R. X. BATISTA; CARLOS A. PEREIRA; RONALDO Z. MENDONÇA; ANGELA M. MORAES. Evaluation of concentrated milk whey as a supplement for SF9 Spodoptera frugiperda cells in culture. ELECTRONIC JOURNAL OF BIOTECHNOLOGY, v. 9, n. 5, p. 0-0, Out. 2006. (02/09482-3)
MENDONÇA‚ R.Z.; GRECO‚ K.N.; SOUSA‚ A.P.B.; MORAES‚ R.H.P.; ASTRAY‚ R.M.; PEREIRA‚ C.A.. Enhancing effect of a protein from Lonomia obliqua hemolymph on recombinant protein production. Cytotechnology, v. 57, n. 1, p. 83-91, 2008. (02/09482-3)
PAMBOUKIAN‚ M.M.; JORGE‚ S.A.C.; SANTOS‚ M.G.; YOKOMIZO‚ A.Y.; PEREIRA‚ C.A.; TONSO‚ A.. Insect cells respiratory activity in bioreactor. Cytotechnology, v. 57, n. 1, p. 37-44, 2008. (02/09482-3)
SWIECH‚ K.; ROSSI‚ N.; ASTRAY‚ R.M.; SUAZO‚ C.A.T.. Enhanced production of recombinant rabies virus glycoprotein (rRVGP) by Drosophila melanogaster S2 cells through control of culture conditions. Cytotechnology, v. 57, n. 1, p. 67-72, 2008. (02/09482-3)
SWIECH‚ K.; ROSSI‚ N.; SILVA‚ B.G.; JORGE‚ S.A.C.; ASTRAY‚ R.M.; SUAZO‚ C.A.T.. Bioreactor culture of recombinant Drosophila melanogaster S2 cells: characterization of metabolic features related to cell growth and production of the rabies virus glycoprotein. Cytotechnology, v. 57, n. 1, p. 61-66, 2008. (02/09482-3)
JORGE‚ S.A.C.; SANTOS‚ A.S.; SPINA‚ Â.; PEREIRA‚ C.A.. Expression of the hepatitis B virus surface antigen in Drosophila S2 cells. Cytotechnology, v. 57, n. 1, p. 51-59, 2008. (02/09482-3)
BATISTA‚ F.R.X.; PEREIRA‚ C.A.; MENDONÇA‚ R.Z.; MORAES‚ A.M.. Enhancement of Sf9 cells and baculovirus production employing Grace’s medium supplemented with milk whey ultrafiltrate. Cytotechnology, v. 49, n. 1, p. 1-9, 2005. (02/09482-3)
GALESI‚ A.L.L.; AGUIAR‚ M.A.; ASTRAY‚ R.M.; AUGUSTO‚ E.F.P.; MORAES‚ Â.M.. Growth of recombinant Drosophila melanogaster Schneider 2 cells producing rabies virus glycoprotein in bioreactor employing serum-free medium. Cytotechnology, v. 57, n. 1, p. 73-81, 2008. (02/09482-3)
BATISTA‚ F.R.X.; PEREIRA‚ C.A.; MENDONÇA‚ R.Z.; MORAES‚ Â.M.. Formulation of a protein-free medium based on IPL-41 for the sustained growth of Drosophila melanogaster S2 cells. Cytotechnology, v. 57, n. 1, p. 11-22, 2008. (02/09482-3)
BATISTA, FABIANA R. X.; GRECO, KATIA N.; ASTRAY, RENATO M.; JORGE, SORAIA A. C.; AUGUSTO, ELISABETH F. P.; PEREIRA, CARLOS A.; MENDONCA, RONALDO Z.; MORAES, ANGELA M.. Behavior of Wild-type and Transfected S2 Cells Cultured in Two Different Media. Applied Biochemistry and Biotechnology, v. 163, n. 1, p. 1-13, JAN 2011. Web of Science Citations: 5. (02/09482-3)
BATISTA, FABIANA R. X.; MORAES, ANGELA M.; BUENTEMEYER, HEINO; NOLL, THOMAS. Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium. BIOLOGICALS, v. 37, n. 2, p. 108-118, APR 2009. Web of Science Citations: 10. (02/09482-3)
NOBRE LEMOS, MARCOS ALEXANDRE; DOS SANTOS, ALEXANDRA SOUZA; ASTRAY, RENATO MANCINI; PEREIRA, CARLOS AUGUSTO; CALIL JORGE, SORAIA ATTIE. Rabies virus glycoprotein expression in Drosophila S2 cells. I: Design of expression/selection vectors, subpopulations selection and influence of sodium butyrate and culture medium on protein expression. Journal of Biotechnology, v. 143, n. 2, p. 103-110, AUG 20 2009. Web of Science Citations: 16. (02/09482-3)
ROSSI, NICKELI; SILVA, BRUNA G.; ASTRAY, RENATO; SWIECH, KAMILLA; PEREIRA, CARLOS A.; SUAZO, CLAUDIO A. T.. Effect of hypothermic temperatures on production of rabies virus glycoprotein by recombinant Drosophila melanogaster S2 cells cultured in suspension. Journal of Biotechnology, v. 161, n. 3, p. 328-335, OCT 31 2012. Web of Science Citations: 6. (02/09482-3)
MORAES, ANGELA M.; JORGE, SORAIA A. C.; ASTRAY, RENATO M.; SUAZO, CLAUDIO A. T.; CALDERON RIQUELME, CAMILO E.; AUGUSTO, ELISABETH F. P.; TONSO, ALDO; PAMBOUKIAN, MARILENA M.; PICCOLI, ROSANE A. M.; BARRAL, MANUEL F.; et al. Drosophila melanogaster S2 cells for expression of heterologous genes: From gene cloning to bioprocess development. BIOTECHNOLOGY ADVANCES, v. 30, n. 3, p. 613-628, MAY-JUN 2012. Web of Science Citations: 33. (02/09482-3)
RODAS‚ V.M.; MARQUES‚ F.H.; HONDA‚ M.T.; SOARES‚ D.M.; JORGE‚ S.A.C.; ANTONIAZZI‚ M.M.; MEDUGNO‚ C.; CASTRO‚ M.E.B.; RIBEIRO‚ B.M.; SOUZA‚ M.L.; et al. Cell culture derived AgMNPV bioinsecticide: biological constraints and bioprocess issues. Cytotechnology, v. 48, n. 1, p. 27-39, 2005. (02/09482-3)
ASTRAY, RENATO M.; VENTINI, DANIELLA C.; BOLDORINI, VERA L. L.; SILVA, FERNANDA G.; ROCCA, MAYRA P.; PEREIRA, CARLOS A.. Rabies virus glycoprotein and immune response pattern using recombinant protein or recombinant RNA viral vectors. Vaccine, v. 32, n. 24, SI, p. 2829-2832, MAY 19 2014. Web of Science Citations: 6. (09/09327-7)
ASTRAY, R. M.; JORGE, S. A. C.; LEMOS, M. A. N.; YOKOMIZO, A. Y.; BOLDORINI, V. L. L.; PUGLIA, A. L. P.; RIBEIRO, O. G.; PEREIRA, C. A.. Kinetic studies of recombinant rabies virus glycoprotein (RVGP) cDNA transcription and mRNA translation in Drosophila melanogaster S2 cell populations. Cytotechnology, v. 65, n. 5, p. 829-838, OCT 2013. Web of Science Citations: 4. (11/08331-0)
VENTINI, DANIELLA C.; DAMIANI, RENATA; SOUSA, ALVARO P. B.; DE OLIVEIRA, JOAO E.; PERONI, CIBELE N.; RIBELA, MARIA T. C. P.; BARTOLINI, PAOLO; TONSO, ALDO; SOARES, CARLOS R. J.; PEREIRA, CARLOS A.. Improved Bioprocess with CHO-hTSH Cells on Higher Microcarrier Concentration Provides Higher Overall Biomass and Productivity for rhTSH. Applied Biochemistry and Biotechnology, v. 164, n. 4, p. 401-409, JUN 2011. Web of Science Citations: 2. (02/09482-3)
VENTINI, DANIELLA CRISTINA; ASTRAY, RENATO MANCINI; NOBRE LEMOS, MARCOS ALEXANDRE; CALIL JORGE, SORAIA ATTIE; CALDERON RIQUELME, CAMILO; TORRES SUAZO, CLAUDIO ALBERTO; TONSO, ALDO; PEREIRA, CARLOS AUGUSTO. Recombinant rabies virus glycoprotein synthesis in bioreactor by transfected Drosophila melanogaster S2 cells carrying a constitutive or an inducible promoter. Journal of Biotechnology, v. 146, n. 4, p. 169-172, APR 15 2010. Web of Science Citations: 16. (02/09482-3)
AUGUSTO, ELISABETH F. P.; MORAES, ANGELA M.; PICCOLI, ROSANE A. M.; BARRAL, MANUEL F.; SUAZO, CLAUDIO A. T.; TONSO, ALDO; PEREIRA, CARLOS A.. Nomenclature and guideline to express the amount of a membrane protein synthesized in animal cells in view of bioprocess optimization and production monitoring. BIOLOGICALS, v. 38, n. 1, p. 105-112, JAN 2010. Web of Science Citations: 8. (02/09482-3)
MENDONCA, RONALDO Z.; GRECO, KATIA N.; MORAES, ROBERTO H. P.; ASTRAY, RENATO M.; BARRAL, M.. Study of kinetic parameters for the production of recombinant rabies virus glycoprotein. Cytotechnology, v. 60, n. 1-3, p. 143-151, JUL 2009. Web of Science Citations: 4. (02/09482-3)
SANTOS, MARIZA G.; JORGE, SORAIA A. C.; BRILLET, KARL; PEREIRA, CARLOS A.. Improving heterologous protein expression in transfected Drosophila S2 cells as assessed by EGFP expression. Cytotechnology, v. 54, n. 1, p. 15-24, May 2007. (02/09482-3)
SUAREZ-PATINO, SANDRA FERNANDA; MANCINI, RENATO ASTRAY; PEREIRA, CARLOS AUGUSTO; TORRES SUAZO, CLAUDIO ALBERTO; MENDONCA, RONALDO ZUCATELLI; CALIL JORGE, SORAIA ATTIE. Transient expression of rabies virus glycoprotein (RVGP) in Drosophila melanogaster Schneider 2 (S2) cells. Journal of Biotechnology, v. 192, n. A, p. 255-262, DEC 20 2014. Web of Science Citations: 5. (02/09482-3, 11/08331-0, 10/08742-8)