| Full text | |
| Author(s): |
Lopes, Andre Moreni
[1]
;
Santos-Ebinuma, Valeria de Carvalho
[2]
;
Apolinario, Alexsandra Conceicao
[3]
;
Bezerra Mendonca, Jr., Francisco Jaime
[3]
;
Goulart de Lima Damasceno, Bolivar Ponciano
[3]
;
Pessoa, Jr., Adalberto
[1]
;
da Silva, Jose Alexsandro
[3]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508 Sao Paulo - Brazil
[2] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Sao Paulo - Brazil
[3] State Univ Paraiba, Grad Program Pharmaceut Sci, Campina Grande - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Process Biochemistry; v. 49, n. 9, p. 1555-1561, SEP 2014. |
| Web of Science Citations: | 5 |
| Abstract | |
Liquid-liquid extraction for the purification of molecules has been central to many advances in the pharmaceutical industry. These processes were developed based on the property that some polymer and/or micellar solutions present to separate into a concentrated phase and a diluted phase. Based on the differences in the physical and chemical environments of the two coexisting phases, and since both phases contain approximately 60-90% of water, liquid-liquid extraction provides a powerful alternative to both extract and solubilize a molecule. This paper examines the partition behavior of the synthetic drug, 2-{[}(3,4-dichlorine-benzylidene)-amino]-5,6-dihydro-4H-cyclopenta{[}b]t hiophene-3-carbonitrile (5CN05), in an aqueous two-phase polymer system (ATPPS) and also in an aqueous two-phase micellar system (ATPMS). The results showed that both systems are favorable for extraction the 5CN05 drug high partition coefficient values (K-5CN05 > 200) and yield (Y-5CN05 > 99.48%) in the concentrated phase were achieved with the systems. However, the ATPPS generated a partition coefficient (K-5CN05) higher than the one obtained with ATPMS. The results suggest that both processes may be used for the extraction and concentration of molecules with hydrophobic characteristics, such as 5CN05. They also provide an optimal environment for the solubilization of such molecules, allowing for greater efficiency when purifying many classes of drugs. (C) 2014 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 13/22212-0 - Extraction, purification and characterization of polysaccharides from Agave sisalana Perrine species targeting different applications in the field of pharmaceutical sciences |
| Grantee: | José Alexsandro da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |