| Full text | |
| Author(s): |
Manoel, Ricardo O.
[1]
;
Freitas, Miguel L. M.
[2]
;
Barreto, Mariana A.
[3]
;
Moraes, Mario L. T.
[1]
;
Souza, Anete P.
[3]
;
Sebbenn, Alexandre M.
[2]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Paulista, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP - Brazil
[2] Inst Florestal Sao Paulo, BR-01059970 Sao Paulo - Brazil
[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | APPLICATIONS IN PLANT SCIENCES; v. 2, n. 3 MAR 2014. |
| Web of Science Citations: | 2 |
| Abstract | |
Premise of the study: Microsatellite primers were developed for the tree species Genipa americana (Rubiaceae) for further population genetic studies. Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)(8) and (GT)(8) motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural G. americana populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively. Conclusions: The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in G. americana (AU) | |
| FAPESP's process: | 11/01518-8 - Contemporary gene flow, intrapopulation spatial genetic structure and hierarchical mating system within and between fruits in a small isolated population of Genipa americana L. using microsatellite markers. |
| Grantee: | Ricardo de Oliveira Manoel |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 10/19613-4 - Pollen dispersal distance and patters and spatial genetic structure in a small isolated population of Genipa americana by microsatellite loco |
| Grantee: | Miguel Luiz Menezes Freitas |
| Support Opportunities: | Regular Research Grants |