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Pollen dispersal distance and patters and spatial genetic structure in a small isolated population of Genipa americana by microsatellite loco

Abstract

The aims of this study are investigate by microssatelite loci the genetic diversity, mating system, gene flow, pollen and seed dispersal land patterns, spatial genetic strture and the effective population size of a small population ( 7.2 ha ) of Genipa americana located in the Estação Ecológica de Mogi-Guaçu, Instituto Florestal de São Paulo. Thus, all 125 adult trees living in the stand will be mapped, measured (diameter at breast height -dbh), sexed and sampled for genetic analysis. To study the seed and pollen dispersal by parentage analysis, 300 seedling will also be mapped, measured and sampled. Open pollinated seeds will be collected from 10 seed trees, 50 seeds per tree, in two consecutive fruiting event (2011 e 2012). From each seed tree, five fruits will be collected (10 seeds per fruit). To investigated the effects of forest fragmentation on descent populations, the genetic diversity, inbreeding, effective population size will be compared between adults, seedlings and offspring. For this purpose, the spatial genetic structure will be also compared between adults and juveniles. The results of this study will permit to understand the contemporary process of mating system after isolation of the population by forest fragmentation and theirs impacts on the effective population size of the descent population. Moreover, the results of this study will permit design strategies for collect seeds for ex situ conservation and environmental restoration. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MANOEL, R. O.; FREITAS, M. L. M.; TAMBARUSSI, E. V.; CAMBUIM, J.; MORAES, M. L. T.; SEBBENN, A. M.. Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae). Genetics and Molecular Research, v. 14, n. 3, p. 8161-8169, . (11/01518-8, 10/19613-4)
MANOEL, RICARDO O.; FREITAS, MIGUEL L. M.; BARRETO, MARIANA A.; MORAES, MARIO L. T.; SOUZA, ANETE P.; SEBBENN, ALEXANDRE M.. DEVELOPMENT AND CHARACTERIZATION OF 32 MICROSATELLITE LOCI IN GENIPA AMERICANA (RUBIACEAE). APPLICATIONS IN PLANT SCIENCES, v. 2, n. 3, . (11/01518-8, 10/19613-4)
MANOEL, R. O.; FREITAS, M. L. M.; FURLANI JUNIOR, E.; ALVES, P. F.; MORAES, M. L. T.; SEBBENN, A. M.. Individual, fruit, and annual variation in correlated mating in a Genipa americana population. SILVAE GENETICA, v. 64, n. 3, p. 108-116, . (11/01518-8, 10/19613-4)

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