Methylation and expression patterns of miRNA and cardiovascular disease and type 2...
| Full text | |
| Author(s): |
Total Authors: 3
|
| Affiliation: | [1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY; v. 293, p. 32-39, NOV 1 2014. |
| Web of Science Citations: | 9 |
| Abstract | |
The photochemistry of safranine complexed with riboflavin and the photoinduced electron transfer from folic acid to the complex were investigated by steady-state and time resolved absorption spectroscopy, and the photoproducts were further analyzed by LC-orbitrap-FT-MS. UV-vis spectra of the complex showed a decrease in the safranine band at 520 nm with increasing concentration of riboflavin, which was attributed to complex formation. A 1:1 (safranine:riboflavin) stoichiometry was determined using Job's method. Following safranine photoexcitation, there is formation of a radical pair in the complex by donation of an electron from ground-state riboflavin to excited-state safranine. The cation radical of riboflavin within this complex reacts with folic acid to yield FA(+){*}, which may accept an electron from the anion radical of safranine, avoiding the photobleaching of the safranine. In contrast, the reaction of the anion radical of non-complexed safranine with folic acid leads to safranine photobleaching. (C) 2014 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 09/54040-8 - Acquisition of a high-resolution Orbitrap mass spectrometer for the discovery and structural analysis of biologically active compounds: applications in proteomics, biomarkers, synthesis, isolation, and characterization of natural products, studies of redox systems in food and enzymatic synthesis |
| Grantee: | Emanuel Carrilho |
| Support type: | Multi-user Equipment Program |
| FAPESP's process: | 12/19823-4 - Phototransients of organoboranes generated by photolysis and flavin sensitization |
| Grantee: | Willy Glen Santos |
| Support type: | Scholarships in Brazil - Post-Doctorate |