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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Leaf-, panel- and latex-expressed sequenced tags from the rubber tree (Hevea brasiliensis) under cold-stressed and suboptimal growing conditions: the development of gene-targeted functional markers for stress response

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Silva, Carla C. [1] ; Mantello, Camila C. [1] ; Campos, Tatiana [2] ; Souza, Livia M. [1] ; Goncalves, Paulo S. [3] ; Souza, Anete P. [4, 1]
Total Authors: 6
[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet CBMEG, BR-13083970 Campinas, SP - Brazil
[2] EMBRAPA, Ctr Pesquisa Agroflorestal Acre CPAFAC, BR-69900970 Rio Branco 69900970, Ac - Brazil
[3] Inst Agronm Campinas IAC, BR-13012970 Campinas, SP - Brazil
[4] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: MOLECULAR BREEDING; v. 34, n. 3, p. 1035-1053, OCT 2014.
Web of Science Citations: 11

Hevea brasiliensis is a native species of the Amazon Basin of South America and the primary source of natural rubber worldwide. Due to the occurrence of South American Leaf Blight disease in this area, rubber plantations have been extended to suboptimal regions. Rubber tree breeding is time-consuming and expensive, but molecular markers can serve as a tool for early evaluation, thus reducing time and costs. In this work, we constructed six different cDNA libraries with the aim of developing gene-targeted molecular markers for the rubber tree. A total of 8,263 reads were assembled, generating 5,025 unigenes that were analyzed; 912 expressed sequence tags (ESTs) represented new transcripts, and two sequences were highly up-regulated by cold stress. These unigenes were scanned for microsatellite (SSR) regions and single nucleotide polymorphisms (SNPs). In total, 169 novel EST-SSR markers were developed; 138 loci were polymorphic in the rubber tree, and 98 % presented transferability to six other Hevea species. Locus duplication was observed in H. brasiliensis and other species. Additionally, 43 SNP markers in 13 sequences that showed similarity to proteins involved in stress response, latex biosynthesis and developmental processes were characterized. cDNA libraries are a rich source of SSR and SNP markers and enable the identification of new transcripts. The new markers developed here will be a valuable resource for linkage mapping, QTL identification and other studies in the rubber tree and can also be used to evaluate the genetic variability of other Hevea species, which are valuable assets in rubber tree breeding. (AU)

FAPESP's process: 07/50562-4 - Development of a genetic linkage map of rubber tree based on microsatellite markers and mapping of loci implied in cold tolerance and other traits of economic importance
Grantee:Anete Pereira de Souza
Support Opportunities: Regular Research Grants
FAPESP's process: 12/50491-8 - Evaluation of allelic variability, population structure and linkage desequilibrium in the rubber tree ex situ germoplasm (Hevea brasiliensis)
Grantee:Anete Pereira de Souza
Support Opportunities: Regular Research Grants
FAPESP's process: 12/05473-1 - Evaluation of the linkage desequilibrium and population structure in rubber tree ex situ germplasm (Hevea brasiliensis) using SNPs (Single Nucleotide Polymorphism)
Grantee:Livia Moura de Souza
Support Opportunities: Scholarships in Brazil - Post-Doctoral