The rubber tree kinome: functional classification and expression patterns in cold stress response

• Grant number: 19/19340-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): November 01, 2019
• Effective date (End): June 30, 2021
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Anete Pereira de Souza
• Grantee: Lucas Borges dos Santos
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The rubber tree (Hevea brasiliensis), a species originally from the Amazon region, is considered as the world's main source of natural rubber, which is the raw material in numerous industrial processes and moves millions of dollars annually. Latex excels from synthetic rubber because of its chemical properties that cannot be replaced, such as elasticity and wear resistance. Although Amazon region is the cradle of the species, its large-scale cultivation is limited in hot and humid climates due to the high incidence of the fungus Pseudocercospora ulei, which causes leaf damage and promotes plant death. As an alternative to the problem, Brazil's heveiculture centers have been moved to "escape" regions, where the different climate conditions such as cold and dry periods, make the fungus development impossible. However, the elaboration of breeding programs for the species was fundamental for the development of new genotypes adapted to this new environment presenting high productivity. The regulation of cellular signaling pathways in response to different abiotic stress conditions, such as temperature, is directly related to the transcription of protein kinase coding genes, which participate in the inhibition or enhancement of metabolic pathways. This study aims to characterize the total set of protein kinase genes encoded by the rubber tree genome, known as kinome, as well as the classification of these proteins into different groups through phylogenetic analysis and the comparison of their expression levels in response to different exposure times at low temperatures (10 C), between two genotypes: GT1 and RRIM600, tolerant and resistant to cold, respectively, in order to understand the mechanisms of regulation of different cell pathways by cold and to provide relevant information for breeding programs that seek the development of genotypes adapted to escape regions having high latex productivity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AONO, ALEXANDRE HILD; GONZAGA PIMENTA, RICARDO JOSE; BASSO GARCIA, ANA LETYCIA; CORRER, FERNANDO HENRIQUE; HOSAKA, GUILHERME KENICHI; CARRASCO, MARISHANI MARIN; CARDOSO-SILVA, CLAUDIO BENICIO; MANCINI, MELINA CRISTINA; SFORCA, DANILO AUGUSTO; DOS SANTOS, LUCAS BORGES; et al. The Wild Sugarcane and Sorghum Kinomes: Insights Into Expansion, Diversification, and Expression Patterns. FRONTIERS IN PLANT SCIENCE, v. 12, JUL 7 2021. (14/11482-9, 18/18588-8, 19/03232-6, 15/22993-7, 15/16399-5, 08/52197-4, 19/21682-9, 05/55258-6, 19/19340-2)