Analysis of RNA expression profiling by RNA-seq in rubber tree and identification of the genes involved in the response to cold

• Grant number: 14/18755-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2014
• Status: Discontinued
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Anete Pereira de Souza
• Grantee: Camila Campos Mantello
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Natural rubber is used to manufacture more than 40,000 products, and plays a fundamental role in the tire industry. Among all species that produce latex, the rubber tree [Hevea brasiliensis (Willd. Ex Adr. Juss.) Muell-Arg.], plant native to the Amazon region, is the largest source of natural rubber in the world. The fungus Microcyclus ulei is the cause of SALB (South American leaf bligth), primary disease that affects rubber tree in Brazil. Although rubber is a plant native of the Amazon region where conditions are optimal for growth and productivity, in this region occurs SALB. Thus, rubber planting expanded to escape areas that present new stress conditions such as wind and lower temperatures and, for this reason, genetic improvement has been seeking clones adapted to these regions. The cycle of genetic improvement of rubber takes approximately 30 years to materialize In this way, it is essential to develop new techniques for early assessment, enabling reduce and optimize ratings for this culture. Techniques such as sequencing of messenger RNA (mRNA) in next-generation sequencing (RNA-seq) platforms have become an important tool, aiding the search for genes responsible for agronomic traits of interest. The RNA-seq gives the transcriptome profile, and is the starting point for the identification of new and / or rare transcripts and is a powerful tool for studying gene expression. Yet, are descriptive transcriptomes rubber obtained by RNA-seq for leaves, latex and panel available, but none of these work aimed to study the gene expression profile under conditions important for breeding programs stress. Thus, this study aims to know the gene expression in response to cold and to identify genes involved in tolerance to this stress as well as identify microsatellite markers (SSRs- Simple Sequence Repeats) and single nucleotide polymorphisms (SNPs) related to genes of interest. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONSON, ANDRE R. O.; TANIGUTI, CRISTIANE H.; AMADEU, RODRIGO R.; ANDREOTTI, ISABELA A. A.; DE SOUZA, LIVIA M.; DOS SANTOS, LUCIANO H. B.; ROSA, JOAO R. B. F.; MANTELLO, CAMILA C.; DA SILVA, CARLA C.; SCALOPPI JUNIOR, ERIVALDO JOSE; et al. High-Resolution Genetic Map and QTL Analysis of Growth-Related Traits of Hevea brasiliensis Cultivated Under Suboptimal Temperature and Humidity Conditions. FRONTIERS IN PLANT SCIENCE, v. 9, AUG 24 2018. (15/24346-9, 12/50491-8, 14/11807-5, 12/05473-1, 17/07908-9, 14/18755-0)

MANTELLO, CAMILA CAMPOS; BOATWRIGHT, LUCAS; DA SILVA, CARLA CRISTINA; SCALOPPI, ERIVALDO JOSE; GONCALVES, PAULO DE SOUZA; BARBAZUK, W. BRAD; DE SOUZA, ANETE PEREIRA. Deep expression analysis reveals distinct cold-response strategies in rubber tree (Hevea brasiliensis). BMC Genomics, v. 20, JUN 4 2019. (14/18755-0, 15/24346-9, 12/50491-8)

DE SOUZA, LIVIA M.; DOS SANTOS, LUCIANO H. B.; ROSA, JOAO R. B. F.; DA SILVA, CARLA C.; MANTELLO, CAMILA C.; CONSON, ANDRE R. O.; SCALOPPI, JR., ERIVALDO J.; FIALHO, JOSEFINO DE F.; DE MORAES, MARIO LUIZ T.; GONCALVES, PAULO DE S.; et al. Linkage Disequilibrium and Population Structure in Wild and Cultivated Populations of Rubber Tree (Hevea brasiliensis). FRONTIERS IN PLANT SCIENCE, v. 9, JUL 3 2018. (12/50491-8, 14/11807-5, 17/07908-9, 14/18755-0, 12/05473-1)