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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Leucine Supplementation Accelerates Connective Tissue Repair of Injured Tibialis Anterior Muscle

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Author(s):
Pereira, Marcelo G. [1] ; Silva, Meiricris T. [1] ; Carlassara, Eduardo O. C. [1] ; Goncalves, Dawit A. [2] ; Abrahamsohn, Paulo A. [3] ; Kettelhut, Isis C. [2] ; Moriscot, Anselmo S. [1] ; Aoki, Marcelo S. [4] ; Miyabara, Elen H. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Physiol & Biochem Immunol, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508000 Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Arts Sci & Humanities, BR-03828000 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: NUTRIENTS; v. 6, n. 10, p. 3981-4001, OCT 2014.
Web of Science Citations: 16
Abstract

This study investigated the effect of leucine supplementation on the skeletal muscle regenerative process, focusing on the remodeling of connective tissue of the fast twitch muscle tibialis anterior (TA). Young male Wistar rats were supplemented with leucine (1.35 g/kg per day); then, TA muscles from the left hind limb were cryolesioned and examined after 10 days. Although leucine supplementation induced increased protein synthesis, it was not sufficient to promote an increase in the cross-sectional area (CSA) of regenerating myofibers (p > 0.05) from TA muscles. However, leucine supplementation reduced the amount of collagen and the activation of phosphorylated transforming growth factor-beta receptor type I (T beta R-I) and Smad2/3 in regenerating muscles (p < 0.05). Leucine also reduced neonatal myosin heavy chain (MyHC-n) (p < 0.05), increased adult MyHC-II expression (p < 0.05) and prevented the decrease in maximum tetanic strength in regenerating TA muscles (p < 0.05). Our results suggest that leucine supplementation accelerates connective tissue repair and consequent function of regenerating TA through the attenuation of T beta R-I and Smad2/3 activation. Therefore, future studies are warranted to investigate leucine supplementation as a nutritional strategy to prevent or attenuate muscle fibrosis in patients with several muscle diseases. (AU)

FAPESP's process: 10/18287-6 - Effect of leucine supplementation on structural and functional recovery of skeletal muscle after injury
Grantee:Eduardo de Oliveira Cruz Carlassara
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 12/24524-6 - Control of muscle mass by cAMP signaling pathway
Grantee:Isis Do Carmo Kettelhut
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/17667-2 - Morfological and molecular skeletal muscle plasticity after injury: effect of high voltage electric stimulation and low potency lasertherapy
Grantee:Elen Haruka Miyabara
Support type: Regular Research Grants
FAPESP's process: 12/15276-9 - Skeletal muscle plasticity after injury in old rats: effect of leucine supplementation
Grantee:Elen Haruka Miyabara
Support type: Regular Research Grants
FAPESP's process: 12/18861-0 - FOXO hyperacetylation as a mechanism of suppression of atrophy gene program induced by beta2-adrenergic signaling in rodent skeletal muscle
Grantee:Dawit Albieiro Pinheiro Gonçalves
Support type: Scholarships in Brazil - Post-Doctorate