Leucine Supplementation Accelerates Connective Tissue Repair of Injured Tibialis Anterior Muscle

Author(s)

Pereira, Marcelo G. ^[1] ; Silva, Meiricris T. ^[1] ; Carlassara, Eduardo O. C. ^[1] ; Goncalves, Dawit A. ^[2] ; Abrahamsohn, Paulo A. ^[3] ; Kettelhut, Isis C. ^[2] ; Moriscot, Anselmo S. ^[1] ; Aoki, Marcelo S. ^[4] ; Miyabara, Elen H. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Physiol & Biochem Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Arts Sci & Humanities, BR-03828000 Sao Paulo - Brazil

• Document type: Journal article
• Source: NUTRIENTS; v. 6, n. 10, p. 3981-4001, OCT 2014.
• Web of Science Citations: 16

Abstract

This study investigated the effect of leucine supplementation on the skeletal muscle regenerative process, focusing on the remodeling of connective tissue of the fast twitch muscle tibialis anterior (TA). Young male Wistar rats were supplemented with leucine (1.35 g/kg per day); then, TA muscles from the left hind limb were cryolesioned and examined after 10 days. Although leucine supplementation induced increased protein synthesis, it was not sufficient to promote an increase in the cross-sectional area (CSA) of regenerating myofibers (p > 0.05) from TA muscles. However, leucine supplementation reduced the amount of collagen and the activation of phosphorylated transforming growth factor-beta receptor type I (T beta R-I) and Smad2/3 in regenerating muscles (p < 0.05). Leucine also reduced neonatal myosin heavy chain (MyHC-n) (p < 0.05), increased adult MyHC-II expression (p < 0.05) and prevented the decrease in maximum tetanic strength in regenerating TA muscles (p < 0.05). Our results suggest that leucine supplementation accelerates connective tissue repair and consequent function of regenerating TA through the attenuation of T beta R-I and Smad2/3 activation. Therefore, future studies are warranted to investigate leucine supplementation as a nutritional strategy to prevent or attenuate muscle fibrosis in patients with several muscle diseases. (AU)

• FAPESP's process: 12/15276-9 - Skeletal muscle plasticity after injury in old rats: effect of leucine supplementation
• Grantee: Elen Haruka Miyabara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/18287-6 - Effect of leucine supplementation on structural and functional recovery of skeletal muscle after injury
• Grantee: Eduardo de Oliveira Cruz Carlassara
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/24524-6 - Control of muscle mass by cAMP signaling pathway
• Grantee: Isis Do Carmo Kettelhut
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 09/17667-2 - Morfological and molecular skeletal muscle plasticity after injury: effect of high voltage electric stimulation and low potency lasertherapy
• Grantee: Elen Haruka Miyabara
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/18861-0 - FOXO HYPERACETYLATION AS A MECHANISM OF SUPPRESSION OF ATROPHY GENE PROGRAM INDUCED BY BETA2-ADRENERGIC SIGNALING IN RODENT SKELETAL MUSCLE
• Grantee: Dawit Albieiro Pinheiro Gonçalves
• Support Opportunities: Scholarships in Brazil - Post-Doctoral