In Vitro Assessment of Mutagenic And Genotoxic Effects of Coumarin Derivatives 6,7-Dihydroxycoumarin and 4-Methylesculetin

Author(s)

Maistro, Edson Luis ^{[1, 2]} ; Marques, Eduardo de Souza ^[1] ; Fedato, Rafael Palhano ^[1] ; Tolentino, Flora ^[2] ; Cezario da Silva, Chayene de Andrade ^[2] ; Ferreira Tsuboy, Marcela Stefanini ^[2] ; Resende, Flavia Aparecida ^[3] ; Varanda, Eliana Aparecida ^[3]

Affiliation

^[1] Univ Estadual Paulista UNESP, Inst Biociencias, Programa Posgrad Biol Geral & Aplicada, Sao Paulo - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Fonoaudiol, Fac Filosofia & Ciencias, Marilia, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Dept Ciencias Biol, Fac Ciencias Farmaceut, Araraquara, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 78, n. 2, p. 109-118, JAN 17 2015.
• Web of Science Citations: 6

Abstract

Coumarins are naturally occurring compounds, widely distributed throughout the plant kingdom (Plantae), and possess important pharmacological properties, including inhibition of oxidative stress. In this context, newly synthesized coumarin compounds are being produced due to their potent antioxidant activities. Therefore, the aim of the present study was to determine the in vitro cytotoxic, mutagenic, and genotoxic effects of 6,7-dihydroxycoumarin (6,7-HC) and 4-methylesculetin (4-ME) using the Salmonella/microsome test and in cultured human lymphocytes the comet assay and micronucleus test. The three coumarin derivatives concentrations evaluated in comet and MN assays were 2, 8, and 32 mu g/mL, selected through a preliminary trypan blue-staining assay. In the Ames test, the 5 concentrations tested were 62.5, 125, 250, 500, and 750 mu g/plate. Positive (methyl methane-sulfonate, MMS) and negative (dimethyl sulfoxide, DMSO) control groups were also included in the analysis. Our results showed that 4-ME induced greater cytotoxicity at high concentrations than 6,7-HC. In addition, both compounds were not mutagenic in the Ames test and not genotoxic or clastogenic/aneugenic in cultured human lymphocytes. (AU)

• FAPESP's process: 12/17241-8 - Investigation of the cytotoxic, genotoxic, antigenotoxic and antioxidant effects of the Euterpe oleracea Mart. (açaí) oil: analysis in vitro and in vivo; histological, ultra-structural and hematological
• Grantee: Edson Luis Maistro
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/07577-3 - Investigation of the genotoxic potential of artesunate, artemisinin, 4-hydroxicumarin and sculetin: in vitro mutagenesis methodology implantation
• Grantee: Edson Luis Maistro
• Support Opportunities: Regular Research Grants