Systemic Host Response Following Skin Burn Injury in Rats: Cytotoxicity and Genotoxicity Evaluation

Author(s)

Quintana, Hananiah Tardivo ^[1] ; Bortolin, Jeferson Andre ^[1] ; Ribeiro, Daniel Araki ^[1] ; De Oliveira, Flavia ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Biosci, BR-11015020 Sao Paulo - Brazil

• Document type: Journal article
• Source: IN VIVO; v. 28, n. 6, p. 1065-1070, NOV-DEC 2014.
• Web of Science Citations: 0

Abstract

Aim: The aim of the present study was to investigate whether skin burn injury (BI) can induce cellular changes in skeletal muscle, liver, kidney and blood by means of DNA damage and cytotoxicity. Materials and Methods: Thirty male Wistar rats were distributed into two groups: control (C) and submitted to scald burn (SB), subdivided into three subgroups: 1, 4 or 14 days post-injury. The gastrocnemius muscle and liver were dissected for histopathological evaluation and the single-cell gel (comet) assay was used to investigate damage in skeletal muscle, liver, kidney and blood cells. Results: Histopathological analysis of the muscle in the SB group revealed congested vessels containing inflammatory cells for all periods evaluated post-injury. In liver, the one day post-injury SB group showed sinusoidal congestion, while that of 14 days post-injury exhibited an increased number of Kupffer cells. Conclusion: Despite the histophatological evidence, none of the groups showed any signs of genotoxicity in these target tissues. (AU)

• FAPESP's process: 11/22034-9 - Morphological, genotoxic and molecular effects of scalding thermal injury in gastrocnemius muscle of young rats
• Grantee: Flávia de Oliveira
• Support Opportunities: Regular Research Grants