Modulation of inflammation response to murine cutaneous Leishmaniosis by homeopathic medicines: Thymulin 5cH

Author(s)

de Santana, Fabiana Rodrigues ^{[1, 2]} ; Coelho, Cideli de Paula ^{[1, 2]} ; Cardoso, Thayna Neves ^[1] ; Laurenti, Marcia Dalastra ^[3] ; Perez Hurtado, Elizabeth Cristina ^[1] ; Bonamin, Leoni Villano ^{[1, 2]}

Affiliation

^[1] Univ Paulista, Res Ctr, BR-04026002 Sao Paulo - Brazil

^[2] Univ Santo Amaro, Lab Vet Pathol, Santo Amaro - Brazil

^[3] Univ Sao Paulo, Fac Med, BR-05508 Sao Paulo - Brazil

• Document type: Journal article
• Source: Homeopathy; v. 103, n. 4, p. 275-284, OCT 2014.
• Web of Science Citations: 9

Abstract

Background: In previous studies, we observed that thymulin 5cH could modulate BCG (Bacillus Calmette-Guerin) induced chronic inflammation by increasing peritoneal B1 stem cells differentiation into phagocytes and improving phagocytosis efficiency. Methods: We used the same protocol to study the effects of thymulin 5cH in the experimental murine Leishmaniasis, in order to elucidate some aspects of the parasite host relation under this homeopathic treatment. Male Balb/c mice were orally treated with thymulin 5cH or vehicle during 60 days, after the subcutaneous inoculation of 2 x 10(6) units of Leishmania (L.) amazonensis into the footpad. Washied inflammatory cell suspension from peritoneal cavity, spleen, local lymph node and infected subcutaneous tissue were harvested after 2 and 60 days from infection to quantify the inflammation cells by flow cytometry and histometry methods. Results: After a transitory increase of peritoneal T reg cells, treated mice presented, chronically, increase in the peritoneal and spleen B1 cells percentage (p = 0.0001) in relation to other cell types; more organized and exuberant inflammation response in the infection site, and decrease in the number of parasites per field inside the primary lesion (p = 0.05). No difference was seen in local lymph node histology. Conclusions: Thymulin 5cH is able to improve B1 cell activation and Leishmania (L) amazonensis phagocytosis efficiency in mice, similarly to that observed previously in BCG experimental infection. (AU)

• FAPESP's process: 10/50842-0 - Effects of thymulin 5cH in BCG-induced granuloma: the B-1 cell participation
• Grantee: Leoni Villano Bonamin
• Support Opportunities: Regular Research Grants