Effectiveness of Fluorescence-Based Methods to Detect in situ Demineralization and Remineralization on Smooth Surfaces

Author(s)

Moriyama, C. M. ^[1] ; Rodrigues, J. A. ^[2] ; Lussi, A. ^[3] ; Diniz, M. B. ^[1]

Affiliation

^[1] Univ Cruzeiro Sul, Cruzeiro Sul Univ, Sch Dent, Dept Pediat Dent, Sao Paulo - Brazil

^[2] Univ Fed Rio Grande do Sul, Fed Univ Rio Grande Sul, Sch Dent, Dept Pediat Dent, Porto Alegre, RS - Brazil

^[3] Univ Bern, Sch Dent Med, Dept Prevent Restorat & Pediat Dent, Bern - Switzerland

• Document type: Journal article
• Source: Caries Research; v. 48, n. 6, p. 507-514, 2014.
• Web of Science Citations: 12

Abstract

This study aimed to evaluate the effectiveness of fluorescence-based methods (DIAGNOdent, LF; DIAGNOdent pen, LFpen, and VistaProof fluorescence camera, FC) in detecting demineralization and remineralization on smooth surfaces in situ. Ten volunteers wore acrylic palatal appliances, each containing 6 enamel blocks that were demineralized for 14 days by exposure to a 20% sucrose solution and 3 of them were remineralized for 7 days with fluoride dentifrice. Sixty enamel blocks were evaluated at baseline, after demineralization and 30 blocks after remineralization by two examiners using LF, LFpen and FC. They were submitted to surface microhardness (SMH) and cross-sectional microhardness analysis. The integrated loss of surface hardness (Delta KHN) was calculated. The intraclass correlation coefficient for interexaminer reproducibility ranged from 0.21 (FC) to 0.86 (LFpen). SMH, LF and LFpen values presented significant differences among the three phases. However, FC fluorescence values showed no significant differences between the demineralization and remineralization phases. Fluorescence values for baseline, demineralized and remineralized enamel were, respectively, 5.4 +/- 1.0, 9.2 +/- 2.2 and 7.0 +/- 1.5 for LF; 10.5 +/- 2.0, 15.0 +/- 3.2 and 12.5 +/- 2.9 for LFpen, and 1.0 +/- 0.0, 1.0 +/- 0.1 and 1.0 +/- 0.1 for FC. SMH and.KHN showed significant differences between demineralization and remineralization phases. There was a negative and significant correlation between SMH and LF and LFpen in the remineralization phase. In conclusion, LF and LFpen devices were effective in detecting demineralization and remineralization on smooth surfaces provoked in situ. (C) 2014 S. Karger AG, Basel (AU)

• FAPESP's process: 10/06855-0 - Performance of fluorescence-based methods in monitoring non cavitated caries-like lesions on smooth surfaces using a bacterial cariogenic model in vitro
• Grantee: Michele Baffi Diniz
• Support Opportunities: Regular Research Grants