Effect of inoculum concentration, pH, light intensity and lighting regime on hydrogen production by phototrophic microbial consortium

Author(s)

Lazaro, Carolina Zampol ^[1] ; Amancio Varesche, Maria Bernadete ^[2] ; Silva, Edson Luiz ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sch Engn Sao Carlos, Dept Hydraul & Sanitat, BR-13566590 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: RENEWABLE ENERGY; v. 75, p. 1-7, MAR 2015.
• Web of Science Citations: 23

Abstract

Photobiological hydrogen production using a bacterial consortium has advantages over pure cultures regarding application to wastewater treatment. Photo-H-2 production from organic acids, which were produced by dark fermentation, must be studied in detail to make a hydrogen production process in two stages efficient. In this scenario, our study aimed to determine the optimized culture conditions for hydrogen production by a phototrophic bacterial consortium. The inoculum concentration, pH, light intensity and illumination protocols were the parameters that we evaluated. The optimal conditions for hydrogen production were inoculum concentration of 0.2 g VSS L-1, pH 7.0, light intensity of 5 klux, with a constant illumination regime. The highest hydrogen production potential (P) and substrate conversion efficiency (SCE) were 41.5 mmol H-2 L-1 culture and 25.4%, respectively, with a COD removal of 95%. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/15984-0 - Bioenergy production from wastewaters and environmental fitting of liquid and solid wastes generated
• Grantee: Marcelo Zaiat
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/03979-5 - Hydrogen production from sugarcane vinasse using sequential two-stage dark and photo fermentation processes
• Grantee: Carolina Zampol Lazaro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral