Electrical Detection of Dengue Biomarker Using Egg Yolk Immunoglobulin as the Biological Recognition Element

Author(s)

Figueiredo, Alessandra ^[1] ; Vieira, Nirton C. S. ^[1] ; dos Santos, Juliana F. ^[1] ; Janegitz, Bruno C. ^[1] ; Aoki, Sergio M. ^[2] ; Junior, Paulo P. ^[2] ; Lovato, Rodrigo L. ^[2] ; Nogueira, Mauricio L. ^[3] ; Zucolotto, Valtencir ^[1] ; Guimaraes, Francisco E. G. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Fis Sao Carlos, Nanomed & Nanotoxicol Grp, BR-13560970 Sao Carlos, SP - Brazil

^[2] DNAPTA Biotecnol, BR-15035470 Sao Jose Do Rio Preto, SP - Brazil

^[3] FAMERP, Dept Doencas Infecciosas & Parasitarias, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 5, JAN 19 2015.
• Web of Science Citations: 23

Abstract

Nonstructural protein 1 (NS1) is secreted by dengue virus in the first days of infection and acts as an excellent dengue biomarker. Here, the direct electrical detection of NS1 from dengue type 2 virus has been achieved by the measurement of variations in open circuit potential (OCP) between a reference electrode and a disposable Au electrode containing immobilized anti-NS1 antibodies acting as immunosensor. Egg yolk immunoglobulin (IgY) was utilized for the first time as the biological recognition element alternatively to conventional mammalian antibodies in the detection of dengue virus NS1 protein. NS1 protein was detected in standard samples in a 0.1 to 10 mg.mL(-1) concentration range with (3.2 +/- 0.3) mV/mu g.mL(-1) of sensitivity and 0.09 mu g.mL(-1) of detection limit. Therefore, the proposed system can be extended to detect NS1 in real samples and provide an early diagnosis of dengue. (AU)

• FAPESP's process: 11/20110-0 - Field effect-based immunosensors to detect myocardial infarction markers
• Grantee: Nirton Cristi Silva Vieira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral