| Full text | |
| Author(s): |
Pizauro, L. J. L.
[1]
;
Silva, D. G.
[1]
;
Santana, A. M.
[1]
;
Clemente, V.
;
Lara, G. H. B.
[2]
;
Listoni, F. J. P.
[2]
;
Vaz, A. C. N.
[3]
;
Vidal-Martins, A. M. C.
[3]
;
Ribeiro, M. G.
[2]
;
Fagliari, J. J.
[1]
Total Authors: 10
|
| Affiliation: | [1] UNESP, FCAV, Jaboticabal, SP - Brazil
[2] UNESP, FMVZ, Botucatu, SP - Brazil
[3] Univ Sao Paulo, FZEA, Pirassununga, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia; v. 66, n. 6, p. 1703-1710, DEC 2014. |
| Web of Science Citations: | 4 |
| Abstract | |
The aim of this study was to evaluate somatic cell count (SCC), prevalence and etiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil, in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile of microorganisms isolated from milk samples was also evaluated. 1,042 milk samples from female Murrah buffaloes in a dairy farm located in Analândia, São Paulo State, Brazil, collected between May 2011 and November 2012 were analyzed. After the mammary gland physical examination, strip cup test and California Mastitis Test (CMT) were performed. Afterwards, 50mL of milk samples from each mammary quarter were collected aseptically for SCC in automatic equipment and microbiological examination. The antimicrobial sensitivity profile to ampicillin, cefoperazone, ceftiofur, enrofloxacin, gentamicin, neomycin, oxacillin, penicillin, and sulfamethoxazole/trimethoprim was evaluated by disk diffusion method. The monthly average temperature and pluviometric index were obtained from "Centro Integrado de Informações Agrometeorológicas" (CIIAGRO) of "Instituto Agronômico de Campinas" (IAC). Milk samples with positive results in the microbiological test showed average SCC of 137,720 cells/mL in the dry period and 190,309 cells/mL in the rainy period. Although a higher number of isolated microorganisms was observed in buffalo milk samples during the rainy period (69/600) compared to the dry period (50/442), the season had no significant effect on the frequency of isolation of microorganisms. The main genera of microorganisms isolated were coagulase-negative Staphylococcus (38.4%), Streptococcus agalactiae(28.8%), and Bacillus spp. (7.56%) during the dry season and Corynebacterium sp. (23.5%), Streptococcus spp. (32.3%), and Streptococcus agalactiae (9.24%) during the rainy period. Multidrug resistance was observed in 30.1% of the isolated microorganisms. (AU) | |
| FAPESP's process: | 11/19480-7 - Biochemical profile, including acute phase proteins and immunoglobulins, microbial condition and somatic cell count in Jaffarabadi primiparous and pluriparous buffaloes over complete lactation |
| Grantee: | José Jurandir Fagliari |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/03514-0 - Biochemical profile, including proteinograme, with emphasis on acute phase proteins and immunoglobulins, microbial condition and somatic cell count in Jaffarabadi buffalo milk primiparous and pluriparous over complete lactation |
| Grantee: | Lucas José Luduverio Pizauro |
| Support Opportunities: | Scholarships in Brazil - Master |