Detection of bla(CTX-M)-type genes in complex class 1 integrons carried by Enterobacteriaceae isolated from retail chicken meat in Brazil

Author(s)

Casella, Tiago ^[1] ; Rodriguez, Maria Margarita ^[2] ; Takahashi, Juliana Tiemi ^[3] ; Ghiglione, Barbara ^[2] ; Dropa, Milena ^[4] ; Assuncao, Ednei ^[5] ; Nogueira, Mauricio Lacerda ^[3] ; Lincopan, Nilton ^{[5, 6]} ; Gutkind, Gabriel ^[2] ; Lelles Nogueira, Mara Correa ^[3]

Affiliation

^[1] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Buenos Aires, Fac Farm & Bioquim, Buenos Aires, BA - Argentina

^[3] Fac Med Sao Jose do Rio Preto, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Saude Publ, Dept Prat Saude Publ, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: International Journal of Food Microbiology; v. 197, p. 88-91, MAR 16 2015.
• Web of Science Citations: 25

Abstract

CIX-M-type extended-spectrum B-lactamase (ESBL)-producing Enterobacteriaceae have been increasingly identified in humans and animals, and their potential transmission by contaminated food has been highlighted. In this study, we report for the first time the isolation of multidrug-resistant (MDR) Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis strains harboring bla(CTXM-2) or bla(CTXM-8) gene variants in chicken meat sold in markets in southeast Brazil. In this regard, the genetic environment of the bla(CTX-M-2) gene is composed of a complex class 1 integron and an ISCR1-associated sequence with dfr and/or aadA gene cassettes located within the variable region. In summary, chicken meat may be a reservoir of MDR Enterobacteriaceae harboring bla(CTX-M)-type genes, which is a public health concern. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/07778-1 - Detection and identification of ESBL and quinolone resistance genes in Salmonella and Escherichia coli isolated from beef, suine and poultry retail meats
• Grantee: Mara Corrêa Lelles Nogueira
• Support Opportunities: Regular Research Grants