Global liver gene expression differences in Nelore steers with divergent residual feed intake phenotypes

Author(s):Show less -

Tizioto, Polyana C. ^{[1, 2]} ; Coutinho, Luiz L. ^[3] ; Decker, Jared E. ^[2] ; Schnabel, Robert D. ^[2] ; Rosa, Kamila O. ^[4] ; Oliveira, Priscila S. N. ^[5] ; Souza, Marcela M. ^[5] ; Mourao, Gerson B. ^[3] ; Tullio, Rymer R. ^[1] ; Chaves, Amalia S. ^[3] ; Lanna, Dante P. D. ^[3] ; Zerlotini-Neto, Adhemar ^[6] ; Mudadu, Mauricio A. ^[1] ; Taylor, Jeremy F. ^[2] ; Regitano, Luciana C. A. ^[1]

Affiliation

^[1] Embrapa Southeast Livestock, Sao Carlos, SP - Brazil

^[2] Univ Missouri, Div Anim Sci, Columbia, MO - USA

^[3] Univ Sao Paulo, ESALQ, Dept Anim Sci, Piracicaba, SP - Brazil

^[4] State Univ Sao Paulo, Dept Anim Sci, Jaboticabal, SP - Brazil

^[5] Univ Fed Sao Carlos, Dept Genet & Evolut, BR-13560 Sao Carlos, SP - Brazil

^[6] Embrapa Agr Informat, Campinas, SP - Brazil

• Document type: Journal article
• Source: BMC Genomics; v. 16, MAR 25 2015.
• Web of Science Citations: 50

Abstract

Background: Efficiency of feed utilization is important for animal production because it can reduce greenhouse gas emissions and improve industry profitability. However, the genetic basis of feed utilization in livestock remains poorly understood. Recent developments in molecular genetics, such as platforms for genome-wide genotyping and sequencing, provide an opportunity to identify genes and pathways that influence production traits. It is known that transcriptional networks influence feed efficiency-related traits such as growth and energy balance. This study sought to identify differentially expressed genes in animals genetically divergent for Residual Feed Intake (RFI), using RNA sequencing methodology (RNA-seq) to obtain information from genome-wide expression profiles in the liver tissues of Nelore cattle. Results: Differential gene expression analysis between high Residual Feed Intake (HRFI, inefficient) and low Residual Feed Intake (LRFI, efficient) groups was performed to provide insights into the molecular mechanisms that underlie feed efficiency-related traits in beef cattle. A total of 112 annotated genes were identified as being differentially expressed between animals with divergent RFI phenotypes. These genes are involved in ion transport and metal ion binding; act as membrane or transmembrane proteins; and belong to gene clusters that are likely related to the transport and catalysis of molecules through the cell membrane and essential mechanisms of nutrient absorption. Genes with functions in cellular signaling, growth and proliferation, cell death and survival were also differentially expressed. Among the over-represented pathways were drug or xenobiotic metabolism, complement and coagulation cascades, NRF2-mediated oxidative stress, melatonin degradation and glutathione metabolism. Conclusions: Our data provide new insights and perspectives on the genetic basis of feed efficiency in cattle. Some previously identified mechanisms were supported and new pathways controlling feed efficiency in Nelore cattle were discovered. We potentially identified genes and pathways that play key roles in hepatic metabolic adaptations to oxidative stress such as those involved in antioxidant mechanisms. These results improve our understanding of the metabolic mechanisms underlying feed efficiency in beef cattle and will help develop strategies for selection towards the desired phenotype. (AU)

• FAPESP's process: 14/13555-3 - Gene expression profiling in hepatic tissue of Nelore steers with divergent profiles for residual feed intake
• Grantee: Polyana Cristine Tizioto
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 13/17778-4 - Tanscriptomic profile of extreme animals for feed efficiency of Nelore breed
• Grantee: Polyana Cristine Tizioto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral