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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Production of exopolysaccharide from rhizobia with potential biotechnological and bioremediation applications

Full text
Author(s):
Luque Castellane, Tereza Cristina [1] ; Persona, Michelli Romanoli [1] ; Campanharo, Joao Carlos [1] ; de Macedo Lemos, Eliana Gertrudes [1]
Total Authors: 4
Affiliation:
[1] UNESP Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 74, p. 515-522, MAR 2015.
Web of Science Citations: 20
Abstract

The potential use of rhizobia under controlled fermentation conditions may result in the production of new extracellular polymeric substances (EPS) having novel and superior properties that will open up new areas of industrial applications and thus increase their demand. The production of EPS and the stability of emulsions formed with soybean oil, diesel oil and toluene using different concentrations of purified EPS derived from wild-type and mutant strains of Rhizobium tropici SEMIA 4077 was investigated. The EPS was defined as a heteropolysaccharide composed of six constituent monosaccharides that displayed higher intrinsic viscosity and pseudoplastic non-Newtonian fluid behavior in an aqueous solution. The ratio between the total EPS production and the cellular biomass was 76.70 for the 4077:Z04 mutant strain and only 8.10 for the wild-type strain. The EPS produced by the wild-type R. tropici SEMIA 4077 resulted in more stable emulsions with the tested toluene than xanthan gum, and the emulsification indexes with hydrocarbons and soybean oil were higher than 50%, indicating strong emulsion-stabilizing capacity. These results demonstrate that the EPS of R. tropici strains could be attractive for use in industrial and environmental applications, as it had higher intrinsic viscosity and good emulsification activity. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/10299-5 - Obtaining mutant gene exoZ exopolysaccharide synthesis in Rhizobium tropici SEMIA 4077.
Grantee:Michelli Romanoli Persona
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/11957-6 - Construction of mutant gene exoz involved in the synthesis of repeating subunits of EPS and the genes phbA, phbB and phbC responsible for the synthesis of PHB in Rhizobium tropici SEMIA 4080
Grantee:Eliana Gertrudes de Macedo Lemos
Support Opportunities: Regular Research Grants