| Full text | |
| Author(s): |
Marconi, Elizabeth C. M.
[1]
;
Bernardes, Nara T. C. G.
[1]
;
Beserra, Laila A. R.
[1]
;
Silva, Fernanda D. F.
[1]
;
Gregori, Fabio
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 35, n. 1, p. 39-43, JAN 2015. |
| Web of Science Citations: | 1 |
| Abstract | |
Group A Rotavirus (RVA) is one of the most common causes of diarrhea in humans and several animal species. A SYBR-Green Real-Time polymerase chain reaction (PCR) was developed to diagnose RVA from porcine fecal samples, targeting amplification of a 137-bp fragment of nonstructural protein 5 (NSP5) gene using mRNA of bovine NADH-desidrogenase-5 as exogenous internal control. Sixty-five samples were tested (25 tested positive for conventional PCR and genetic sequencing). The overall agreement (kappa) was 0.843, indicating 'very good' concordance between tests, presenting 100% of relative sensitivity (25+ Real Time PCR/25+ Conventional PCR) and 87.5% of relative sensitivity (35- Real Time PCR/40- Conventional PCR). The results also demonstrated high intra- and inter-assay reproducibility (coefficient of variation ≤1.42%); thus, this method proved to be a fast and sensitive approach for the diagnosis of RVA in pigs. (AU) | |
| FAPESP's process: | 11/10846-9 - Development of a real time PCR method for porcine rotavirus diagnosis |
| Grantee: | Fábio Gregori |
| Support Opportunities: | Regular Research Grants |