Bacterial communities in thermophilic H-2-producing reactors investigated using 16S rRNA 454 pyrosequencing

Author(s)

Ratti, Regiane Priscila ^[1] ; Delforno, Tiago Palladino ^[1] ; Okada, Dagoberto Yukio ^[1] ; Amancio Varesche, Maria Bernadete ^[1]

Affiliation

^[1] Univ Sao Paulo, BR-13563120 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: MICROBIOLOGICAL RESEARCH; v. 173, p. 10-17, 2015.
• Web of Science Citations: 11

Abstract

In this study, the composition and diversity of the bacterial community in thermophilic H-2-producing reactors fed with glucose were investigated using pyrosequencing. The H-2-producing experiments in batch were conducted using 0.5 and 2.0 gl(-1) glucose at 550 degrees C. Under the two conditions, the H-2 production and yield were 1.3 and 1.6 mol H-2 mol glucose(-1), respectively. Acetic, butyric, iso-butyric, lactic and propionic acids were detected in the two reactors. The increase in substrate concentration favored a high H-2 yield. In this reactor, a predominance of acetic and iso-butyric acids, 27.7% and 40%, were measured, respectively. By means of pyrosequencing, a total of 323 and 247 operational taxonomic units were obtained, with a predominance of the phylum Firmicutes (68.73-67.61%) for reactors with 0.5 and 2.0 gl(-1) glucose, respectively. Approximately 40.55% and 62.34% of sequences were affiliated with Thermoanaerobacterium and Thermohydrogenium, microorganisms that produce H-2 under thermophilic conditions. (C) 2015 Elsevier GmbH. All rights reserved. (AU)

• FAPESP's process: 10/01237-6 - Hydrolisis of sugar cane bagasse for hydrogen production in thermophilic condition
• Grantee: Regiane Priscila Ratti Sartori
• Support Opportunities: Scholarships in Brazil - Post-Doctoral