Developing a common bean core collection suitable for association mapping studies

Author(s)

Kuepper Cardoso Perseguini, Juliana Morini ^{[1, 2]} ; Borges Silva, Gliciane Micaele ^[2] ; Bachega Feijo Rosa, Joao Ricardo ^[3] ; Gazaffi, Rodrigo ^[4] ; Marcal, Jessica Fernanda ^[2] ; Morais Carbonell, Sergio Augusto ^[5] ; Chiorato, Alisson Fernando ^[5] ; Zucchi, Maria Imaculada ^[6] ; Franco Garcia, Antonio Augusto ^[3] ; Benchimol-Reis, Luciana Lasry ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao & Bioagentes, BR-13012970 Campinas, SP - Brazil

^[2] Inst Agron Campinas, Ctr Recursos Genet Vegetais, Campinas, SP - Brazil

^[3] Escola Super Agr Luis Dequeiroz, Dept Genet, Piracicaba, SP - Brazil

^[4] Univ Fed Sao Carlos, Ctr Ciencias Agr, Dept Biotecnol Vegetal, Araras, SP - Brazil

^[5] Inst Agron Campinas, Ctr Graos & Fibras, Campinas, SP - Brazil

^[6] Agencia Paulista Tecnol Agronegocios, Inst Agron, Ctr Desenvolvimento Tecnol, Campinas, SP - Brazil

• Document type: Journal article
• Source: GENETICS AND MOLECULAR BIOLOGY; v. 38, n. 1, p. 67-78, 2015.
• Web of Science Citations: 9

Abstract

Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the Structure analysis. According to analysis of molecular variance, the most divergent accessions comprised 97.2% of the observed genetic variability present within the base collection, confirming the efficiency of the selection criterion. The 180 selected accessions will be used for association mapping in future studies and could be potentially used by breeders to direct new crosses and generate elite cultivars that meet current and future global market needs. (AU)

• FAPESP's process: 09/02502-8 - Linkage desequilibrium study in Phaseolus vulgaris L. to perform associative mapping of biotic and abiotic traits.
• Grantee: Juliana Morini Küpper Cardoso Perseguini
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/05284-1 - Linkage desequilibrium study in Phaseolus vulgaris L. for associative mapping of biotic and abiotic traits
• Grantee: Luciana Lasry Benchimol-Reis
• Support Opportunities: Regular Research Grants